What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?

R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts.

Does the Luminex Rat Magnetic Assay detect soluble form or membrane-bound analyte?

The sample types typically validated by R&D Systems for Luminex assays contain soluble form of proteins, and therefore it is expected that the soluble form of proteins are detected. It is possible there are assays that also detect the membrane form of respective target proteins, however, R&D Systems does not generally evaluate our assays against samples known to contain membrane-bound proteins.

Rat Luminex® Discovery Assay

Name: Rat Luminex® Discovery Assay
Brand: R&D Systems
SKU: LXSARM
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # LXSARM

Citations (21)

(1)

Product Datasheet / COA / SDS

Luminex Discovery Assays

Luminex Discovery Assays provide the most flexible assays on the market. These panels are completely customizable using our large menu of human, mouse, and rat analytes, making them the perfect tool for biomarker discovery and screening.

Build your own Luminex Assay with our Luminex Assay Customization Tool.

Build Now

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Magnetic bead-based multiplex assay for the Luminex® platform
Format:	1 x 96-well microplate and magnetic antibody-coated beads
Species:	Rat
Analytes Detected:	Select from over 15 analytes to build your custom panel using our assay customization tool below.

View Full Technical Details for Rat Luminex® Discovery Assay

Product Features for Rat Luminex® Discovery Assay

Magnetic Luminex Assays are the most flexible bead-based multiplex assays that we offer. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples.

Simultaneously profile up to 50 analytes of your choice in one sample

Choose from a wide menu of analytes, including many unique analytes
Requires a small sample volume (<50 µL)
Run your assay in just 3-4 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate
Quick turn-around time: Kits are typically shipped within 5-10 days of ordering.*

Please allow additional time for international orders.

Performance Validation for Rat Luminex® Discovery Assay

Luminex Assays are validated for use with cell culture supernatants, plasma and serum. All Luminex assays are tested for sensitivity, intra-assay precision, inter-assay precision and to ensure assay linearity for validated sample types. Antibody pairs are selected and tested to confirm the parallel detection of natural and recombinant standard protein and to ensure the accurate determination of target analytes within biological samples. Assays for each target analyte are screened against all target analytes to confirm low antibody cross-reactivity.

Kit Components for Rat Luminex® Discovery Assay

Premixed cocktail of antibody-coated Magnetic beads
Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Build Your Assay

Make Your Selections Below to Configure Your Custom Luminex Assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	•Store the unopened kit at 2-8 °C. Do not use past the expiration date on the label. • Avoid freezing microparticles. • Protect microparticles from light.

Product Specific Notices for Rat Luminex® Discovery Assay

Luminex® is a registered trademark of Luminex Corporation.

Citations for Rat Luminex® Discovery Assay (21)

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Citations are publications that use Bio-Techne products. Selected citations for Rat Luminex® Discovery Assay include:

Species: Rat
Sample Types: Tissue Homogenates

NA Lopes et al. (2022), Social Isolation Stress Modulates Pregnancy Outcomes and the Inflammatory Profile of Rat Uterus International Journal of Molecular Sciences, 23(11).
PMID: 35682846
Species: Rat
Sample Types: Serum

E Liljedahl et al. (2022), Long-term anti-tumor effects following both conventional radiotherapy and FLASH in fully immunocompetent animals with glioblastoma Scientific Reports, 12(1):12285.
PMID: 35853933
Species: Mouse
Sample Types: Tissue Homogenates

MF Barbe et al. (2021), Key indicators of repetitive overuse-induced neuromuscular inflammation and fibrosis are prevented by manual therapy in a rat model Bmc Musculoskeletal Disorders, 22(1):417.
PMID: 33952219
Species: Rat
Sample Types: Tissue Homogenates

BK Schilling et al. (2021), Intramuscular injection of skeletal muscle derived extracellular matrix mitigates denervation atrophy after sciatic nerve transection Journal of tissue engineering, 12(0):2041731421103.
PMID: 34567507
Species: Rat
Sample Types: Serum

D Lee et al. (2020), Commiphora Extract Mixture Ameliorates Monosodium Iodoacetate-Induced Osteoarthritis Nutrients, 12(5).
PMID: 32438772
Species: Rat
Sample Types: Plasma

MR Amorim et al. (2020), Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation Sci Rep, 10(1):6990.
PMID: 32332859
Species: Rat
Sample Types: Plasma

HJ Lee et al. (2020), Ginseng-Induced Changes to Blood Vessel Dilation and the Metabolome of Rats Nutrients, 12(8).
PMID: 32727012
Species: Rat
Sample Types: Cell Culture Supernates

T He et al. (2020), Trauma induced tissue survival in vitro with a muscle-biomaterial based osteogenic organoid system: a proof of concept study BMC Biotechnol., 20(1):8.
PMID: 32005149
Species: Rat
Sample Types: CSF

ND Le et al. (2020), Evaluation of neurofilament light chain in the cerebrospinal fluid and blood as a biomarker for neuronal damage in experimental pneumococcal meningitis J Neuroinflammation, 17(1):293.
PMID: 33028339
Species: Rat
Sample Types: Cell Culture Supernates

X Li et al. (2020), Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats Int J Nanomedicine, 15(0):5645-5659.
PMID: 32848391
Species: Rat
Sample Types: Cell Culture Supernates

L Muri et al. (2020), Repetitive transcranial magnetic stimulation activates glial cells and inhibits neurogenesis after pneumococcal meningitis PLoS ONE, 15(9):e0232863.
PMID: 32915781
Species: Rat
Sample Types: Serum

X Li et al. (2020), Anti-Inflammatory Effects of Magnetically Targeted Mesenchymal Stem Cells on Laser-Induced Skin Injuries in Rats Int J Nanomedicine, 15(0):5645-5659.
PMID: 32848391
Species: Rat
Sample Types: Plasma

G Drouin et al. (2019), Comparative effects of dietary n-3 docosapentaenoic acid (DPA), DHA and EPA on plasma lipid parameters, oxidative status and fatty acid tissue composition J. Nutr. Biochem., 63(0):186-196.
PMID: 30412907
Species: Rat
Sample Types: Serum

L Jing et al. (2019), Cardiovascular toxicity of decabrominated diphenyl ethers (BDE-209) and decabromodiphenyl ethane (DBDPE) in rats Chemosphere, 223(0):675-685.
PMID: 30802833
Species: Rat
Sample Types: Tissue Homogen

DK Sung et al. (2019), Thrombin Preconditioning of Extracellular Vesicles Derived from Mesenchymal Stem Cells Accelerates Cutaneous Wound Healing by Boosting Their Biogenesis and Enriching Cargo Content J Clin Med, 8(4).
PMID: 31003433
Species: Rat
Sample Types: CSF

L Muri et al. (2019), Metformin mediates neuroprotection and attenuates hearing loss in experimental pneumococcal meningitis J Neuroinflammation, 16(1):156.
PMID: 31351490
Species: Rat
Sample Types: Cell Culture Supernates

F Evaristo-M et al. (2019), Preconditioning of Rat Bone Marrow-Derived Mesenchymal Stromal Cells with Toll-Like Receptor Agonists Stem Cells Int, 2019(0):7692973.
PMID: 31531025
Species: Rat
Sample Types: Plasma

P Krashia et al. (2019), Blunting neuroinflammation with resolvin D1 prevents early pathology in a rat model of Parkinson's disease Nat Commun, 10(1):3945.
PMID: 31477726
Species: Rat
Sample Types: Tissue Homogenates

MR Amorim et al. (2019), Neuroinflammation in the NTS is associated with changes in cardiovascular reflexes during systemic inflammation J Neuroinflammation, 16(1):125.
PMID: 31221164
Species: Rat
Sample Types: Tissue Homogenates

C Isaac et al. (2017), Mycolactone displays anti-inflammatory effects on the nervous system PLoS Negl Trop Dis, 11(11):e0006058.
PMID: 29149212
Species: Rat
Sample Types: Tissue Homogenates

GM Bove et al. (2016), Manual therapy as an effective treatment for fibrosis in a rat model of upper extremity overuse injury J. Neurol. Sci, 361(0):168-80.
PMID: 26810536

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