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Rat Luminex® Discovery Assay

Bio-Techne includes R&D Systems | Catalog # LXSARM

Luminex Discovery Assays

Luminex Discovery Assays provide the most flexible assays on the market. These panels are completely customizable using our large menu of human, mouse, and rat analytes, making them the perfect tool for biomarker discovery and screening.

Build your own Luminex Assay with our Luminex Assay Customization Tool.

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Key Product Details

Assay Type: Magnetic bead-based multiplex assay for the Luminex® platform
Format: 1 x 96-well microplate and magnetic antibody-coated beads
Species: Rat
Analytes Detected: Select from over 15 analytes to build your custom panel using our assay customization tool below.

Citations for Rat Luminex® Discovery Assay (28)

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  • Rat Luminex® Discovery Assay
    Name: Flavia Millesi
    Sample Tested: Olfactory enshathing cells,Schwann cells
    Verified Customer | Posted 08/02/2023
    Rat Luminex® Discovery Assay LXSARM
  • Rat Luminex® Discovery Assay
    Name: Anonymous
    Sample Tested: Recombinant protein
    Verified Customer | Posted 11/20/2021
    in vitro work with rat recombinant protein

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FAQs for Rat Luminex® Discovery Assay

  • Are QC controls available for both the Luminex Discovery and High Performance Assays?

    Controls are not offered for Luminex Discovery Assays, but may be formulated by the end user. A general guide may be available upon request. Controls are available for select Luminex High Performance Assays and can be found under the supplemental products tab on the product webpage. Some High Performance Luminex kits include controls and the controls will be listed in the materials provided section on the product datasheet. When included in the kit, controls are not sold separately.

  • What is the reason for the recommendation to use 35 µL sample volume, instead of the default 50 µL volume, for Luminex multiplex containing > 25 analytes, when assayed on Luminex MAGPIX® analyzer?

    R&D Systems recommend 35 µL sample volume for > 25 analytes to control the number of beads in the sample load when using Luminex MAGPIX® analyzer. Luminex MAGPIX® analyzer is a non-flow-based reader which utilizes a magnet to hold the magnetic microparticles in a monolayer in a chamber where the beads are imaged and then processed by the software to generate data. Since MAGPIX® system pushes the entire Sample Load Volume into the chamber, multiplex assay containing > 25 analytes may overload the chamber capacity with 50 µL sample load default setting. The captured images can have too many beads in them to give accurate results, and some of the analytes/regions may present lower than desired bead counts. 

  • Does the Luminex Rat Magnetic Assay detect soluble form or membrane-bound analyte?

    The sample types typically validated by R&D Systems for Luminex assays contain soluble form of proteins, and therefore it is expected that the  soluble form of proteins are detected. It is possible there are assays that also detect the membrane form of  respective target proteins, however, R&D Systems does not generally evaluate our assays against samples known to contain membrane-bound proteins.

  • Why does the Luminex Assay Customization Tool provide the option to split based on dilution?

    The feature to split based on dilution enables investigators to configure premix Luminex panels based on sample type (cell culture supernates, serum, and plasma). This keeps analytes requiring the same dilution factor within a single panel, while separating analytes requiring different dilutions. The recommended dilution factor for each analyte may be different for each sample type, and the suggested sample dilutions are built into the Luminex Customization Tool. Note that the suggested dilution factors are based on samples from healthy volunteers.

  • Is the reconstituted standard a concentrate?

    For Discovery Assays, each standard cocktail is a concentrate upon reconstitution with the volume specified on the Certificate of Analysis (CoA). After creating Standard 1 following the table in the product insert, the Standard 1 value is the concentration listed on the CoA.

Product Documents for Rat Luminex® Discovery Assay

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.