NHP XL Cytokine Luminex® Performance Premixed Kit

Name: NHP XL Cytokine Luminex® Performance Premixed Kit
Brand: R&D Systems
SKU: FCSTM21
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FCSTM21

Citations (2)

(3)

Product Datasheet / COA / SDS

Luminex High Performance Assays

Luminex High Performance Assays go through rigorous development, validation, and quality control testing to ensure you are getting the best performance out of your multiplex assay. Panels are customizable so you can order a selection of the analytes or the entire panel.

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Setting the standard in quality research reagents

Key Product Details

Panel:	Luminex Performance NHP XL Cytokine Panel
Assay Type:	Magnetic bead-based multiplex assay for the Luminex^® platform
Format:	1 x 96-well microplate and magnetic antibody-coated beads
Species:	Primate
Analytes Detected:	BDNF CCL2/JE/MCP-1 CCL5/RANTES CCL11/Eotaxin CCL20/MIP-3 alpha CD40 Ligand/TNFSF5 CXCL2/GRO beta/MIP-2/CINC-3 CXCL10/IP-10/CRG-2 CXCL11/I-TAC CXCL13/BLC/BCA-1 FGF basic/FGF2/bFGF G-CSF GM-CSF Granzyme B IFN-alpha IFN-beta IFN-gamma IL-1 beta/IL-1F2 IL-2 IL-4 IL-5 IL-6 IL-7 IL-8/CXCL8 IL-10 IL-12 p70 IL-13 IL-15 IL-17/IL-17A IL-21 PD-L1/B7-H1 PDGF-AA PDGF-BB TGF-alpha TNF-alpha VEGF

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services .

View Full Technical Details for NHP XL Cytokine Luminex® Performance Premixed Kit

Product Features for NHP XL Cytokine Luminex® Performance Premixed Kit

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile up to 36 analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation for NHP XL Cytokine Luminex® Performance Premixed Kit

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Non-human Primate XL validation data on rndsystems.com.

Kit Components for NHP XL Cytokine Luminex® Performance Premixed Kit

Premixed cocktail of antibody-coated Magnetic beads
Premixed cocktail of biotinylated detection antibody
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**
High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 31 different serum assays.
	n=40 (20x2 levels)	n=31
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
BDNF	8.16	13.3
CCL2/MCP-1	3.02	10.5
CCL5/RANTES	3.72	17.0
CCL11/Eotaxin	7.98	15.4
CCL20/MIP-3 alpha	8.41	17.3
CD40 Ligand	9.30	15.0
CXCL2/GRO beta	7.76	13.1
CXCL10/IP-10	2.95	12.2
CXCL11/I-TAC	6.23	13.7
CXCL13/BLC	5.79	12.5
FGF basic	5.60	13.1
G-CSF	5.55	14.2
GM-CSF	7.11	14.1
Granzyme B	9.76	18.6
IFN-alpha	5.17	12.4
IFN-beta	10.9	15.2
IFN-gamma	6.36	13.0
IL-1 beta	2.55	12.7
IL-10	8.55	14.1
IL-12 p70	4.92	17.7
IL-13	7.97	17.5
IL-15	4.99	18.2
IL-17A	4.38	19.0
IL-2	5.32	18.1
IL-21	5.84	19.0
IL-4	4.92	17.5
IL-5	4.14	16.5
IL-6	6.80	17.8
IL-7	6.57	17.7
IL-8/CXCL8	6.87	17.5
PDGF-AA	6.52	25.0
PDGF-BB	3.37	16.9
PD-L1	8.36	19.4
TGF-alpha	5.43	18.7
TNF-alpha	3.68	17.2
VEGF	4.79	18.4

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
	Linearity	Spiked Recovery
Analyte	Average %	Average %
BDNF	114	HE
CCL2/MCP-1	75	135
CCL5/RANTES	98	HE
CCL11/Eotaxin	93	138
CCL20/MIP-3 alpha	105	111
CD40 Ligand	73	105
CXCL2/GRO beta	95	81
CXCL10/IP-10	78	72
CXCL11/I-TAC	NO	97
CXCL13/BLC	NO	114
FGF basic	NV
G-CSF	NO	67
GM-CSF	NO	111
Granzyme B	NO	122
IFN-alpha	NO	118
IFN-beta	NO	71
IFN-gamma	NO	124
IL-1 beta	NO	68
IL-10	NO	104
IL-12 p70	NO	109
IL-13	NO	118
IL-15	NO	90
IL-17A	NO	90
IL-2	NO	89
IL-21	NO	121
IL-4	NO	80
IL-5	NO	95
IL-6	NO	84
IL-7	NO	97
IL-8/CXCL8	121	HE
PDGF-AA	101	99
PDGF-BB	103	HE
PD-L1	80	HE
TGF-alpha	NV
TNF-alpha	NO	85
VEGF	NO	97

Build Your Assay

Make Your Selections Below to Configure Your Custom Luminex Assay

Assays

Below are kits that are available based on your selections. These selections may be further customized before adding to the cart or emailing the configuration to your purchasing department or local distributor to place your order. Selected analytes may be included in more than one High Performance Assay. All assay options are displayed.

If you run into bead region incompatibilities, send your Luminex code to our team and find out more.

How would you like these analytes mixed?

Premixed

User Mixed

Please select at least one mixing option to see the kits.

Would you like to split based on dilution?

Do not split

Split based on serum

Split based on plasma

Split based on cell culture supernates

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Scientific Data Examples for NHP XL Cytokine Luminex® Performance Premixed Kit

	NHP Luminex Standard Curves (Cell Culture Supernates)

Preparation and Storage

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Specific Notices for NHP XL Cytokine Luminex® Performance Premixed Kit

Luminex® is a registered trademark of Luminex Corporation.

Citations for NHP XL Cytokine Luminex® Performance Premixed Kit (2)

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Citations are publications that use Bio-Techne products. Selected citations for NHP XL Cytokine Luminex® Performance Premixed Kit include:

Species: Macaca leonina
Sample Types: Serum

M Milani et al. (2022), Liver-directed lentiviral gene therapy corrects hemophilia A mice and achieves normal-range factor VIII activity in non-human primates Nature Communications, 13(1):2454.
PMID: 35508619
Species: Rhesus Macaque
Sample Types: Cell Culture Supernates

JM Scordo et al. (2022), Recall responses in the lung environment are impacted by age in a pilot study of Mycobacterium bovis-BCG vaccinated rhesus macaques Experimental gerontology, 167(0):111904.
PMID: 35918043

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Customer Reviews for NHP XL Cytokine Luminex® Performance Premixed Kit (3)

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