Does Catalog # FCSTM18 contain Thimerasol?

Name: Human XL Cytokine Luminex® Performance Panel Premixed Kit
Brand: R&D Systems
SKU: FCSTM18
Rating: 5 (9 reviews)

This kit does not contain Thimerasol. R&D Systems transitioned away from this preservative in 2008. Proclin 300 is now used.

Human XL Cytokine Luminex® Performance Panel Premixed Kit

Catalog # FCSTM18 | R&D Systems, Inc. a Bio-Techne Brand

(9)

Citations (18)

Product Datasheet / COA / SDS

New Version Available - FCSTM18B

Discontinued Product

FCSTM18 has been discontinued and is replaced by FCSTM18B.

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Magnetic bead-based multiplex assay for the Luminex® platform
Format:	96-well microplate and antibody-coated magnetic beads*
Species:	Human

Not finding your analyte of interest? View our complete Luminex offering in the full version of our Luminex Assay Customization Tool. We also offer Custom Luminex Services .

View Full Technical Details for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Technical Data
Citations (18) / Reviews (9) / FAQs

Product Features for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Magnetic Luminex High Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

Simultaneously profile Human PD-L1/B7-H1 with up to -1 analytes of your choice in one sample
Magnetic format allows for easier wash steps
Undergoes similar validation testing as Quantikine® ELISA Kits
Precise and Specific
Requires a small sample volume (<50 µL)
Run your assay in just 3-5 hours
Mass-calibrated standards for consistent results with every new lot of material
Enough reagents for one 96-well plate

Performance Validation for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Our Luminex High Performance Assays undergo our most extensive validation testing. All Luminex High Performance Assay Panels are validated for use with serum and plasma samples. Some High Performance Assays are additionally validated for cell culture supernatants, milk, saliva, or urine, as indicated on the individual product data sheets. Luminex High Performance Assays are tested for sensitivity (three-quarters the low standard), intra-assay precision, inter-assay precision, and to ensure assay linearity for validated sample types. Recovery values for individual samples in validated sample types are also tested. Validation data for each analyte can be found in the product datasheet.

View Luminex High Performance Human XL validation data.

Kit Components for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent
Wash Buffer
Streptavidin-PE
One flat-bottom 96-well Microplate
Foil Plate Sealers (4)
Mixing Bottle
Standard Value Card**

**A standard curve must be generated each time an assay is run, utilizing values from the Certificate of Analysis provided with each Luminex Assay.

Precision

Intra-Assay Precision - is generated from the mean of the %CV's from 40 reportable results across two different concentrations of analytes in a single serum assay Inter-Assay Precision - is generated from the mean of the %CV's across two different concentrations of analytes across 25 different serum assays.
	n=40 (20x2 levels)	n=25
Analyte	Intra-Assay (%CV)	Inter-Assay (%CV)
B7-H1/PD-L1	1.8%	11.3%
CCL11/Eotaxin	3.2%	10.0%
CCL19/MIP-3beta	2.2%	9.7%
CCL2/MCP-1	1.4%	8.9%
CCL20/MIP-3 alpha	2.4%	9.9%
CCL3/MIP-1alpha	1.5%	7.3%
CCL4/MIP-1beta	3.0%	9.6%
CD40 Ligand	3.3%	10.6%
CXCL1/GRO alpha	1.0%	8.0%
CXCL10/IP-10	2.1%	8.8%
CXCL2/GRO beta	1.2%	8.8%
CXCL8/IL-8	1.8%	9.0%
EGF	3.0%	9.6%
FGF-basic	4.2%	12.6%
Flt-3 ligand	2.5%	10.1%
G-CSF	1.8%	10.2%
GM-CSF	3.1%	10.3%
Granzyme B	1.8%	11.0%
IFN-alpha	2.9%	9.9%
IFN-beta	3.0%	11.0%
IFN-gamma	2.8%	10.4%
IL-10	3.3%	9.0%
IL-12 p70	2.3%	10.5%
IL-13	1.6%	9.7%
IL-15	2.4%	9.9%
IL-17A	2.5%	11.2%
IL-17E/IL-25	1.7%	9.2%
IL-1alpha	3.0%	10.4%
IL-1beta	2.3%	10.0%
IL-1ra	2.1%	10.3%
IL-2	2.2%	9.2%
IL-3	2.5%	10.1%
IL-33	2.4%	12.3%
IL-4	3.2%	9.7%
IL-5	2.2%	12.0%
IL-6	2.6%	9.8%
IL-7	2.5%	9.8%
PDGF-AA	2.8%	15.5%
PDGF-AB/BB	2.4%	12.4%
RANTES	4.7%	17.4%
TGF-alpha	3.0%	9.8%
TNF-alpha	2.2%	8.7%
TRAIL	2.0%	10.3%
VEGF	2.6%	10.3%

Accuracy

Linearity - The data represents mean spiked or natural linearity in serum matrix samples. Samples with natural linearity are marked with an asterisk. Spike Recovery - The data represents mean percent recovery of spiked standards ranging from low, medium, and high concentration in serum matrix samples.
	Linearity		Spiked Recovery
Analyte	N	Average %	N	Average %
*Natural linearity
B7-H1/PD-L1	4	106%	4	109%
CCL11/Eotaxin	4	92%	4	107%
CCL19/MIP-3beta	4	123%	4	95%
CCL2/MCP-1	4*	82%		High endogenous
CCL20/MIP-3 alpha	4	107%	4	99%
CCL3/MIP-1alpha	4	77%	4	139%
CCL4/MIP-1beta	4	103%	4	111%
CD40 Ligand	4	113%	4	100%
CXCL1/GRO alpha	4	80%		High endogenous
CXCL10/IP-10	3	110%		High endogenous
CXCL2/GRO beta	4*	114%	3	106%
CXCL8/IL-8	4	118%	4	96%
EGF	4*	110%	4	106%
FGF-basic	4	161%	3	80%
Flt-3 ligand	4	102%	4	109%
G-CSF	4	209%	4	43%
GM-CSF	4	96%	4	110%
Granzyme B	4	99%	4	118%
IFN-alpha	3	98%	4	101%
IFN-beta	4	148%	4	69%
IFN-gamma	4	97%	4	112%
IL-10	4	111%	4	104%
IL-12 p70	4	106%	4	108%
IL-13	4	112%	4	103%
IL-15	3	88%	3	89%
IL-17A	4	110%	4	106%
IL-17E/IL-25	4	122%	4	87%
IL-1alpha	4	143%	4	80%
IL-1beta	4	107%	4	99%
IL-1ra	4*	100%	4	145%
IL-2	4	105%	4	102%
IL-3	4	123%	4	88%
IL-33	4	93%	4	110%
IL-4	3	127%	3	77%
IL-5	3	113%	3	81%
IL-6	3	124%	3	90%
IL-7	4	113%	4	96%
PDGF-AA	4*	90%		High endogenous
PDGF-AB/BB	4*	96%		High endogenous
RANTES	4*	96%		High endogenous
TGF-alpha	4	117%	4	96%
TNF-alpha	4	117%	4	69%
TRAIL	3	123%	4	88%
VEGF	4	103%	4	109%

Which Luminex Assay is Right For Me?

Assays for the Luminex platform are offered as High Performance Assays or Discovery Assays . The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available as Configurable User Mix and Premixed options or as Fixed Panels. The Luminex Discovery Assays allow for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays on rndsystems.com .

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using a Luminex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a FLEXMAP 3D dual laser, flow-based systems.

Scientific Data Examples for Human XL Cytokine Luminex® Performance Panel Premixed Kit

	Human XL Cytokine Luminex Panel Standard Curves
	Human XL Cytokine Performance Luminex Panel Serum Sample Values Serum samples from 20 apparently healthy individuals were run on the Human XL Cytokine Luminex High Performance Panel and sample values plotted. Minimum and maximum values shown by exterior lines, the box represents the 1st and 3rd quartiles with the center line representing the median.

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Product Specific Notices for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Luminex® is a registered trademark of Luminex Corporation.

Citations for Human XL Cytokine Luminex® Performance Panel Premixed Kit (18)

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Citations are publications that use Bio-Techne products. Selected citations for Human XL Cytokine Luminex® Performance Panel Premixed Kit include:

Species: Human
Sample Types: Serum

M Chaszczews et al. (2023-01-19), The Influence of Comorbidities on Chemokine and Cytokine Profile in Obstructive Sleep Apnea Patients: Preliminary Results Journal of Clinical Medicine, 2023-012(3).
PMID: 36769452
Species: Human
Sample Types: Serum

A Madè et al. (2022-11-21), Association of miR-144 levels in the peripheral blood with COVID-19 severity and mortality Scientific Reports, 2022-112(1):20048.
PMID: 36414650
Species: Human
Sample Types: Serum

ML Sarangam et al. (2022-09-07), Intestinal Injury Biomarkers Predict Mortality in Pediatric Severe Malaria MBio, 2022-00(0):e0132522.
PMID: 36069443
Species: Human
Sample Types: Cell Culture Supernates

S Shapira et al. (2022-07-13), A novel platform for attenuating immune hyperactivity using EXO-CD24 in Covid-19 and beyond Oncogene, 2022-00(0):e15997.
PMID: 35776000
Species: Human
Sample Types: Serum

A Gandini et al. (2022-07-13), Intestinal fatty acid binding protein (I-FABP) and CXC3L1 evaluation as biomarkers for patients at high-risk for coeliac disease in Johannesburg, South Africa Cytokine, 2022-0157(0):155945.
PMID: 35841826
Species: Human
Sample Types: Cell Culture Supernates

Z Bai et al. (2022-04-05), Combining adoptive NK cell infusion with a dopamine-releasing peptide reduces senescent cells in aged mice Cell Death Disease, 2022-04-05;13(4):305.
PMID: 35383143
Species: Human
Sample Types: Serum

T Gajewski et al. (2021-11-24), Severe COVID-19 infection is associated with aberrant cytokine production by infected lung epithelial cells rather than by systemic immune dysfunction Research square, 2021-10(0).
PMID: 34845442
Species: Human
Sample Types: Plasma

M Barathan et al. (2021-10-05), Comparative expression of pro-inflammatory and apoptotic biosignatures in chronic HBV-infected patients with and without liver cirrhosis Microbial pathogenesis, 2021-1161(0):105231.
PMID: 34619310
Species: Human
Sample Types: Cell Culture Supernates

MT Rogers et al. (2021-06-09), A high-throughput microfluidic bilayer co-culture platform to study endothelial-pericyte interactions Scientific Reports, 2021-011(1):12225.
PMID: 34108507
Species: Human
Sample Types: Serum

Y Tan et al. (2021-06-08), Integrating longitudinal clinical laboratory tests with targeted proteomic and transcriptomic analyses reveal the landscape of host responses in COVID-19 Cell Discovery, 2021-07(1):42.
PMID: 34103487
Species: Human
Sample Types: Cell Culture Supernates

S Ramu et al. (2021-06-02), Direct effects of mast cell proteases, tryptase and chymase, on bronchial epithelial integrity proteins and anti-viral responses BMC immunology, 2021-022(1):35.
PMID: 34078278
Species: Human
Sample Types: Serum

L Yan et al. (2021-03-19), Role of serum CXCL9 and CXCL13 in predicting infection after kidney transplant: A STROBE study Medicine, 2021-0100(11):e24762.
PMID: 33725942
Species: Human
Sample Types: Serum

MK Cerny et al. (2021-03-05), Wound fluid under occlusive dressings from diabetic patients show an increased angiogenic response and fibroblast migration Journal of tissue viability, 2021-00(0).
PMID: 33707159
Species: Human
Sample Types: Cell Culture Supernates

V Khanna et al. (2021-02-08), Novel TLR 7/8 agonists for improving NK cell mediated antibody-dependent cellular cytotoxicity (ADCC) Scientific Reports, 2021-011(1):3346.
PMID: 33558639
Species: Human
Sample Types: CSF

XY Jiang et al. (2020-08-08), Co-expression of NMDA-receptor subunits NR1, NR2A, and NR2B in dysplastic neurons of teratomas in patients with paraneoplastic NMDA-receptor-encephalitis: a retrospective clinico-pathology study of 159 patients Acta Neuropathol Commun, 2020-08(1):130.
PMID: 32771066
Species: Human
Sample Types: Serum

A Wiedemann et al. (2020-07-24), Long-lasting severe immune dysfunction in Ebola virus disease survivors Nat Commun, 2020-011(1):3730.
PMID: 32709840
Species: Human
Sample Types: Aqueous Humor

B Lu et al. (2019-12-03), Multiple cytokine analyses of aqueous humor from the patients with retinitis pigmentosa Cytokine, 2019-1127(0):154943.
PMID: 31810025
Species: Human
Sample Types: Cell Culture Supernates

M Moreno-Vil et al. (2018-11-21), Synergistic Effects of Weightlessness, Isoproterenol, and Radiation on DNA Damage Response and Cytokine Production in Immune Cells Int J Mol Sci, 2018-119(11).
PMID: 30469384

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Customer Reviews for Human XL Cytokine Luminex® Performance Panel Premixed Kit (9)

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Human XL Cytokine Luminex Performance Panel Premixed Kit

Name: Anonymous

Sample Tested: Serum and Plasma

Verified Customer | Posted 11/13/2020
Human XL Cytokine Luminex Performance Panel Premixed Kit

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 11/09/2020
Human XL Cytokine Luminex Performance Panel Premixed Kit

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 10/16/2020
Luminex Performance Assay Human XL Cytokine Discovery Panel

Name: Anonymous

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Verified Customer | Posted 04/21/2020

CRS in house method development using PBMCs.
Luminex Performance Assay Human XL Cytokine Discovery Panel

Name: Anonymous

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Verified Customer | Posted 04/21/2020

CRS assay method development
Luminex Performance Assay Human XL Cytokine Discovery Panel

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 04/21/2020

T cell cytokine release signature with mAb stimulation.
Luminex Performance Assay Human XL Cytokine Discovery Panel

Name: Anonymous

Sample Tested: Cell culture supernatant

Verified Customer | Posted 04/21/2020

T cell responses when co-incubate with a cancer cell with mAb stimulation.
Luminex Performance Assay Human XL Cytokine Discovery Panel

Name: Kyu Hong

Sample Tested: Serum

Verified Customer | Posted 04/21/2020

screening a disease and healthy donor serum
Luminex Performance Assay Human XL Cytokine Discovery Panel

Name: Anonymous

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Verified Customer | Posted 04/21/2020

Cytokine Release Assay using healthy donor PBMC stimulated with different mAbs

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Showing 1 - 5 of 9 reviews Showing All

FAQs for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Does Catalog # FCSTM18 contain Thimerasol?

This kit does not contain Thimerasol. R&D Systems transitioned away from this preservative in 2008. Proclin 300 is now used.

Human XL Cytokine Luminex® Performance Panel Premixed Kit

Catalog # FCSTM18 | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Product Features for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Performance Validation for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Kit Components for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Precision

Accuracy

Which Luminex Assay is Right For Me?

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Scientific Data Examples for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Preparation and Storage

Product Specific Notices for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Citations for Human XL Cytokine Luminex® Performance Panel Premixed Kit (18)

Customer Reviews for Human XL Cytokine Luminex® Performance Panel Premixed Kit (9)

Customer Images

Human XL Cytokine Luminex Performance Panel Premixed Kit

Human XL Cytokine Luminex Performance Panel Premixed Kit

Human XL Cytokine Luminex Performance Panel Premixed Kit

Luminex Performance Assay Human XL Cytokine Discovery Panel

Luminex Performance Assay Human XL Cytokine Discovery Panel

Luminex Performance Assay Human XL Cytokine Discovery Panel

Luminex Performance Assay Human XL Cytokine Discovery Panel

Luminex Performance Assay Human XL Cytokine Discovery Panel

Luminex Performance Assay Human XL Cytokine Discovery Panel

FAQs for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Product Documents for Human XL Cytokine Luminex® Performance Panel Premixed Kit

Product Datasheets

COA

Certificate of Analysis

SDS

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