Rat IgG1 Isotype Control

Detection of Rat IgG Control by Flow Cytometry

Mouse platelets were stained with Rat Anti-Mouse GPVI Monoclonal Antibody (Catalog # MAB6758, filled histogram) or Rat IgG Isotype Control Antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog F0113).

Detection of IgG1 by Immunohistochemistry

Blockade of STAT3 activation reduces IRIADAWestern blot analysis shows phosphorylated STAT3 (p‐Stat3), Erk1 and Erk2 (p‐Erk1/2), and beta‐Actin in the skin of WT, IL‐6−/−, and IL‐1R−/− mice at indicated times (days) post‐IR (15 Gy).BHistochemical (H&E) and immunostaining (red) staining of T cells (CD3+), neutrophils (Ly6b+), and phosphorylated Stat3 (p‐Stat3; red nuclear staining, black arrows) in skin thin sections prepared at 14 days post‐IR from mice treated by daily topical application of aquosum (control), ruxolitinib, or tofacitinib following irradiation (15 Gy). Scale bar, 100 μm. (CD3 and Ly6b immunostaining images from Tofacitinib‐treated mice represent separate analyses performed on serial thin sections.)CQuantification of epidermal thickness, T cells (CD3+) and neutrophils (Ly6b+), and p‐STAT3+ cells in (B), (n = 4).DPhotographic images showing hair loss in mice at 14 days post‐IR (15 Gy) following treatment by daily topical application of aquosum (carrier control) or JAK inhibitors, ruxolitinib, or tofacitinib. Chest and neck hairs of mice in all groups were clipped prior to irradiation to enhance drug absorption.EQuantification of the area of ventral hair loss (in pixels) in mice in (D), (n = 4).Data information: Data represent mean ± SD. *P < 0.05, **P < 0.01, by two‐tailed Student's t‐test; two independent experiments (See also Appendix Fig S8).Source data are available online for this figure. Image collected and cropped by CiteAb from the following open publication (https://link.springer.com/10.15252/emmm.202115653), licensed under a CC-BY license. Not internally tested by R&D Systems.