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Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27231AF700

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NBP2-27231AF700
Conjugate
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry

Label

Alexa Fluor 700 (Excitation = 675-700 nm, Emission = 723 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # MPC-11

Concentration

Please contact technical services for concentration.

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700] [NBP2-27231AF700]

Flow Cytometry: Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700] [NBP2-27231AF700]

Flow Cytometry: Mouse IgG2B Isotype Control (MPC-11) [Alexa Fluor 700] [NBP2-27231AF700] - A surface stain was performed on hPBMCs with CCR1 (53504) antibody FAB145N and a matched isotype control NBP2-27231AF700. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NB100-77758AF488.

Applications for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700]

Application
Recommended Usage

Flow Cytometry

1 ul/1 million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NBP2-27231. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50 mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please contact technical services for concentration.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Mouse IgG2b Isotype Control (MPC-11) [Alexa Fluor® 700]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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