Mouse IgA Kappa Isotype Control (MAK)

Mouse

ELISA, Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, SDS-Page, Western Blot

Unconjugated

Monoclonal Mouse IgA Clone # MAK

Purified

1.0 mg/ml

Mouse IgA Kappa

Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgG3, IgM. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.

Monoclonal

Mouse

IgA

This isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgA alpha and anti-Mouse serum.

Store vial at 4C prior to opening. Stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Multi-step

0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0

Purified

0.1% Sodium Azide

1.0 mg/ml

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at 4C short term. For extended storage, add an equal volume of glycerol, aliquot and store at -20C or below. Avoid repeated freeze-thaw cycles.