Thalidomide-Cyanine 5
Tocris Bioscience, part of Bio-Techne | Catalog # 7288
High affinity cereblon fluorescent probe
Key Product Details
Description
High affinity cereblon fluorescent probe
Product Description
Thalidomide-Cyanine 5 is a fluorescent probe for cereblon E3 ligases. Binds with high affinity to DDB1-CRBN. Suitable for use in TR-FRET.Optical Data
| Emission Color | Red |
| λabs | 650 nm |
| λem | 665 nm |
| Closest Laser line | 647 nm |
| Application | TR-FRET |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Molecular Weight
1041.20
Formula
C52H60N6O13S2
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
2-((1E,3E)-5-((E)-1-(6-((4-(2-((2-(2,6-Dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)acetamido)butyl)amino)-6-oxohexyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
InChI Key
RWTYSFWRLGGPLR-UHFFFAOYSA-N
SMILES
O=C1N(C2CCC(NC2=O)=O)C(C3=CC=CC(OCC(NCCCCNC(CCCCCN(C(C=CC(S(=O)(O)=O)=C4)=C4C/5(C)C)C5=C\C=C\C=C\C6=[N+](CC)C7=C(C=C(S(=O)([O-])=O)C=C7)C6(C)C)=O)=O)=C31)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 20.82 | 20 |
Preparing Stock Solutions
for Thalidomide-Cyanine 5
The following data is based on the product molecular weight 1041.20
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 4.80 mL | 24.01 mL | 48.02 mL |
| 1 mM | 0.96 mL | 4.80 mL | 9.60 mL |
| 2 mM | 0.48 mL | 2.40 mL | 4.80 mL |
| 10 mM | 0.10 mL | 0.48 mL | 0.96 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Chessum Demonstrating in-cell target engagement using a pirin protein degradation probe (CCT367766). J.Med.Chem. 2018 PMID: 29240418
- Petzold Structural basis of lenalidomide-induced CK1α degradation by the CRL4CRBN ubiquitin ligase. Nature 2016 PMID: 26909574
- Cavadini Cullin-RING ubiquitin E3 ligase regulation by the COP9 signalosome. Nature 2016 PMID: 27029275
- Fischer Structure of the DDB1-CRBN E3 ubiquitin ligase in complex with thalidomide. Nature 2014 PMID: 25043012
Product Specific Notices for Thalidomide-Cyanine 5
For research use only
Loading...
Loading...