Sulfidefluor 7 AM
Tocris Bioscience, part of Bio-Techne | Catalog # 4943
H2S fluorescent probe
Key Product Details
Description
H2S fluorescent probe
Product Description
Sulfidefluor 7 AM is a reported to be a hydrogen sulphide (H2S) fluorescent probe. Contains an acetoxymethyl ester, which enhances cell permeability and is hydrolyzed inside the cell to trap the probe. Used to visualize VEGF-induced H2S production in human umbilical vein endothelial cells (HUVECS). Excitation wavelength ~ 495 nm; emission maximum ~ 520 nm.Licensing Information
Sold under license from the Regents of the University of CaliforniaOptical Data
| Emission Color | Green |
| λabs | 495 nm |
| λem | 520 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy |
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Product Specifications
Molecular Weight
685.55
Formula
C31H23N7O12
Storage
Store at -20°C
Chemical Name
N-[2-[(Acetyloxy)methoxy]-2-oxoethyl]-N-[(3',6'-diazido-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]glycine (acetyloxy)methyl ester
CAS Number
1416872-50-8
PubChem ID
89306941
InChI Key
QIAZHSBWGCYJGQ-UHFFFAOYSA-N
SMILES
O=C(O1)C2=C(C=CC(C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O)=C2)C31C4=CC=C(N=[N+]=[N-])C=C4OC5=C3C=CC(N=[N+]=[N-])=C5
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 13.71 | 20 |
Preparing Stock Solutions
for Sulfidefluor 7 AM
The following data is based on the product molecular weight 685.55
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.2 mM | 7.29 mL | 36.47 mL | 72.93 mL |
| 1 mM | 1.46 mL | 7.29 mL | 14.59 mL |
| 2 mM | 0.73 mL | 3.65 mL | 7.29 mL |
| 10 mM | 0.15 mL | 0.73 mL | 1.46 mL |
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Background References
References are publications that support the biological activity of the product.
- Lippert Reaction-based fluorescent probes for selective imaging of hydrogen sulfide in living cells. J.Am.Chem.Soc. 2011 PMID: 21671682
- Lin Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production. Proc.Natl.Acad.Sci.USA. 2013 PMID: 23589874
Product Specific Notices for Sulfidefluor 7 AM
For research use only
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