SiRA 2
Tocris Bioscience, part of Bio-Techne | Catalog # 7544
Fluorogen for SiRA light-up aptamer. Application: confocal microscopy, SRM (including STED)
Key Product Details
Description
Fluorogen for SiRA light-up aptamer. Application: confocal microscopy, SRM (including STED)
Product Description
Key information: SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells.Application: confocal microscopy, super resolution microscopy (SRM) techniques, including super-resolution stimulated emission depletion (STED). Cell permeable.
Properties and Photophysical Data: SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Excitation and emission maxima (λ) are 649 nM and 662 nM, respectively; quantum yield = 0.98; extinction coefficient = 86,000 M-1cm-1; KD = 403 nM.
Reconstitute in 200 μL for a 0.5 mM stock solution. A typical working concentration for this product is 500 nM, see references provided for more details.
We recommend using 5mM Mg2+ (MgCl2 or MgSO4) in all buffers/media for maximum fluorescence intensity.
Licensing Information
Sold with kind permission of Prof. Andres JäschkeOptical Data
| Emission Color | Far Red |
| λabs | 649 nm |
| λem | 662 nm |
| Extinction Coefficient (ε) | 86000 M-1cm-1 |
| Quantum Yield (φ) | 0.98 |
| Closest Laser line | 640 nm |
| Cell Permeable | Yes |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
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Product Specifications
Molecular Weight
602.81
Formula
C33H42N4O5Si
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
(5-((2-(2-(2-Aminoethoxy)ethoxy)ethyl)carbamoyl)-2-(7-(dimethylamino)-3-(dimethyliminio)-5,5-dimethyl-3,5-dihydrodibenzo[b,e]silin-10-yl)benzoate)
CAS Number
2365417-91-8
PubChem ID
168440271
InChI Key
VZACMAUVTBSQSR-UHFFFAOYSA-N
SMILES
CN(C1=CC=C2C([Si](C)(C3=C/C(C=CC3=C2C4=C(C([O-])=O)C=C(C(NCCOCCOCCN)=O)C=C4)=[N+](C)\C)C)=C1)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 30.14 | 50 | |
| DMSO | 60.28 | 100 |
Preparing Stock Solutions
for SiRA 2
The following data is based on the product molecular weight 602.81
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.66 mL | 8.29 mL | 16.59 mL |
| 5 mM | 0.33 mL | 1.66 mL | 3.32 mL |
| 10 mM | 0.17 mL | 0.83 mL | 1.66 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.33 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Wirth SiRA: A silicon rhodamine-binding aptamer for live-cell super-resolution RNA imaging. J.Am.Chem.Soc. 2019 PMID: 30986047
Product Specific Notices for SiRA 2
For research use only
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