SCOTfluor lactic acid probe 510
Tocris Bioscience, part of Bio-Techne | Catalog # 7448
Fluorescent lactic acid probe
Key Product Details
Description
Fluorescent lactic acid probe
Product Description
SCOTfluor lactic acid probe 510 is a red fluorescent probe for imaging lactic acid metabolism in vivo. Excitation and emission maxima (λ) are 485 and 605 nm, respectively. Suitable for use with flow cytometry and super-resolution microscopy.Scientific Data Images for SCOTfluor lactic acid probe 510
Application of SCOTfluor lactic acid probe 510 in HeLa cells.
TIRF microscopy image (left) of HeLa cells after incubation with SCOTfluor 510 lactate (100 μM). Multi-tracking of fluorescent particles corresponding to SCOTfluor 510 lactate labelling (right). Scale: 5 μm. Image kindly provided by Prof. Marc Vendrell, acquired by Dr Mathew Horrocks, School of Chemistry, University of Edinburgh.Licensing Information
Sold under license from the University of EdinburghOptical Data
| Emission Color | Orange |
| λabs | 485 nm |
| λem | 605 nm |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Super Resolution Microscopy (SRM), Flow Cytometry, in vivo |
Spectra Viewer
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Product Specifications
Molecular Weight
331.15
Formula
C9H8N4O5Se
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(2S)-2-Hydroxy-3-[(7-nitro-2,1,3-benzoselenadiazol-4-yl)amino]propanoic acid
CAS Number
2351933-31-6
PubChem ID
146168509
InChI Key
HQRYGRFCRSBEJR-LURJTMIESA-N
SMILES
O[C@H](C(O)=O)CNC1=CC=C(N(=O)=O)C2=N[Se]N=C12
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 3.31 | 10 |
Preparing Stock Solutions
for SCOTfluor lactic acid probe 510
The following data is based on the product molecular weight 331.15
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 30.20 mL | 150.99 mL | 301.98 mL |
| 0.5 mM | 6.04 mL | 30.20 mL | 60.40 mL |
| 1 mM | 3.02 mL | 15.10 mL | 30.20 mL |
| 5 mM | 0.60 mL | 3.02 mL | 6.04 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Monsorno Loss of microglial MCT4 leads to defective synaptic pruning and anxiety-like behavior in mice. Nat.Commun. 2023 PMID: 37717033
- Benson SCOTfluors: Small, conjugatable, orthogonal, and tunable fluorophores for in vivo imaging of cell metabolism. Angew.Chem Int.Ed.Engl. 2019 PMID: 30924239
Product Specific Notices for SCOTfluor lactic acid probe 510
For research use only
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