SCOTfluor glucose probe 510
Tocris Bioscience, part of Bio-Techne | Catalog # 7447
Fluorescent glucose probe
Key Product Details
Description
Fluorescent glucose probe
Product Description
SCOTfluor glucose probe 510 is a fluorescent probe for visualizing glucose uptake in vivo. It can be multiplexed with BFP and GFP. Excitation and emission maxima (λ) are 490 and 605 nm, respectively.Scientific Data Images for SCOTfluor glucose probe 510
Application of SCOTfluor glucose probe 510 in HeLa cells.
Fluorescence microscopy image of GLUT4-EGFP HeLa cells treated with SCOTfluor glucose probe 510 (100 μm). Colors: green (GLUT4-EGFP), red (SCOTfluor glucose probe 510), blue: Hoechst 33342 (Catalog # 5117). Image kindly provided by Prof. Marc Vendrell, acquired by Dr Sam Benson, Centre for Inflammation Research, University of Edinburgh.
Application of SCOTfluor glucose probe 510 in zebrafish embryos.
In vivo images of zebrafish embryos (28 h post fertilization) after injection of SCOTfluor glucose probe 510 (50 pmol) to the yolk sac in wildtype zebrafish (left) or zebrafish injected at one cell stage with 4.2 ng anti-sense glu2 morpholino (right). Image kindly provided by Prof. Marc Vendrell, acquired by Dr Yi Feng, Centre for Inflammation Research, University of Edinburgh.Licensing Information
Sold under license from the University of EdinburghOptical Data
| Emission Color | Orange |
| λabs | 490 nm |
| λem | 605 nm |
| Closest Laser line | 488 nm |
| Cell Permeable | Yes |
| Application | Fluorescence Microscopy, Confocal Microscopy, in vivo Imaging |
Spectra Viewer
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Product Specifications
Molecular Weight
405.23
Formula
C12H14N4O7Se
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2-Deoxy-2-[(7-nitro-2,1,3-benzoselenadiazol-4-yl)amino]-D-glucose
CAS Number
2490298-72-9
PubChem ID
155694629
InChI Key
LXWDEVJSKWMYFZ-BGCUHRRXSA-N
SMILES
OC[C@H]1OC([C@@H]([C@H]([C@@H]1O)O)NC2=CC=C(N(=O)=O)C3=N[Se]N=C23)O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 4.05 | 10 |
Preparing Stock Solutions
for SCOTfluor glucose probe 510
The following data is based on the product molecular weight 405.23
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 24.68 mL | 123.39 mL | 246.77 mL |
| 0.5 mM | 4.94 mL | 24.68 mL | 49.35 mL |
| 1 mM | 2.47 mL | 12.34 mL | 24.68 mL |
| 5 mM | 0.49 mL | 2.47 mL | 4.94 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Benson Photoactivatable metabolic warheads enable precise and safe ablation of target cells in vivo. Nat.Commun. 2021 PMID: 33888691
- Benson SCOTfluors: small, conjugatable, orthogonal, and tunable fluorophores for in vivo imaging of cell metabolism. Angew.Chem.Int.Ed.Engl. 2019 PMID: 30924239
Product Specific Notices for SCOTfluor glucose probe 510
For research use only
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