Rf470DL
Tocris Bioscience, part of Bio-Techne | Catalog # 7406
Blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Key Product Details
Description
Blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria
Product Description
Rf470DL is a blue rotor-fluorogenic fluorescent D-amino acid for labeling peptidoglycans in live bacteria. Rf470DL fluoresces only when it becomes incorporated into the peptidoglycan, enabling no wash experiments. Excitation/emission λ ~470/620 nm; quantum yield = 0.042; extinction coefficient 33,106 M-1 cm-1 (measured in PBS containing 50% glycerol).Licensing Information
Sold under license from Indiana University. U.S. patent application number 16/967,874.Optical Data
| Emission Color | Red |
| λabs | 470 nm |
| λem | 640 nm |
| Extinction Coefficient (ε) | 33106 M-1cm-1 |
| Quantum Yield (φ) | 0.042 |
| Application | Fluorogenic Probe for Imaging Bacterial Cell Wall Biosynthesis (PG Biosynthesis) |
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Product Specifications
Molecular Weight
547.07
Formula
C26H30N4O5S.HCl
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
N6-[3-[6-[5-[2-Carboxy-2-cyanoethenyl]-2-thienyl]-3,4-dihydro-1(2H)-quinolinyl]-1-oxopropyl]-D-lysine hydrochloride
InChI Key
ITTDOARQMPIHGH-IVUCCOTMSA-N
SMILES
N[C@@H](C(O)=O)CCCCNC(CCN1CCCC2=CC(C3=CC=C(/C=C(C(O)=O)\C#N)S3)=CC=C12)=O.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 54.71 | 100 |
Preparing Stock Solutions
for Rf470DL
The following data is based on the product molecular weight 547.07
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.83 mL | 9.14 mL | 18.28 mL |
| 5 mM | 0.37 mL | 1.83 mL | 3.66 mL |
| 10 mM | 0.18 mL | 0.91 mL | 1.83 mL |
| 50 mM | 0.04 mL | 0.18 mL | 0.37 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Hsu Fluorogenic D-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays. Nat.Chem. 2019 PMID: 30804500
Product Specific Notices for Rf470DL
For research use only
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