Peroxy Orange 1
Tocris Bioscience, part of Bio-Techne | Catalog # 4944
Fluorescent hydrogen peroxide indicator
Key Product Details
Description
Fluorescent hydrogen peroxide indicator
Alternative Names
PO1
Product Description
Peroxy Orange 1 is a fluorescent probe for imaging hydrogen peroxide (H2O2); cell permeable. Displays an orange intracellular fluorescence in response to H2O2 signals produced in RAW 264.7 macrophages and A431 cells during immune response and growth factor stimulation, respectively. Excitation wavelength 543 nm, emission between 545 and 750 nm.Licensing Information
Sold under license from the Regents of the University of CaliforniaSpectra Viewer
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Product Specifications
Molecular Weight
521.41
Formula
C32H32BNO5
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
2',3',6',7'-Tetrahydro-12'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-spiro[isobenzofuran-1(3H),9'-[1H,5H,9H]xantheno[2,3,4-ij]quinolizin]-3-one
CAS Number
1199576-10-7
PubChem ID
44546671
InChI Key
KNTNXZHHCPNNGB-UHFFFAOYSA-N
SMILES
O=C(O1)C2=C(C=CC=C2)C31C4=CC5=C6C(CCCN6CCC5)=C4OC7=C3C=CC(B8OC(C)(C)C(C)(C)O8)=C7
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 5.21 | 10 |
Preparing Stock Solutions
for Peroxy Orange 1
The following data is based on the product molecular weight 521.41
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 19.18 mL | 95.89 mL | 191.79 mL |
| 0.5 mM | 3.84 mL | 19.18 mL | 38.36 mL |
| 1 mM | 1.92 mL | 9.59 mL | 19.18 mL |
| 5 mM | 0.38 mL | 1.92 mL | 3.84 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Dickinson Chemistry and biology of reactive oxygen species in signaling or stress responses. Nat.Chem.Biol. 2011 PMID: 21769097
- Woolley H2O2 production downstream of FLT3 is mediated by p22phox in the endoplasmic reticulum and is required for STAT5 signalling. PLoS ONE 2012 PMID: 22807997
- Dickinson A palette of fluorescent probes with varying emission colors for imaging hydrogen peroxide signaling in living cells. J.Am.Chem.Soc. 2010 PMID: 20361787
Product Specific Notices for Peroxy Orange 1
For research use only
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