PARPi-FL
Tocris Bioscience, part of Bio-Techne | Catalog # 6461
Potent fluorescent PARP inhibitor; cell permeable
Key Product Details
Description
Potent fluorescent PARP inhibitor; cell permeable
Alternative Names
AZD2281-BDY FL,Olaparib-BDY FL
Product Description
PARPi-FL is a fluorescent potent PARP inhibitor (IC50 = 12 nM). Composed of a PARP inhibitor conjugated to BDY FL, SE (Cat. No. 5465). Colocalizes with PARP immunostaining in multiple cancer cell lines in vivo and accumulates in tumor cells and tumor associated macrophages. Enables high temporal and subcellular spatial resolution of drug distribution. Emission max = 525 nm. Cell permeable.Optical Data
| Emission Color | Green |
| λabs | 502 nm |
| λem | 510 nm |
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Product Specifications
Molecular Weight
640.46
Formula
C34H32BF3N6O3
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
(T-4)-[4-[[3-[[4-[3-[5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrol-2-yl-κN-1-oxopropyl]-1-piperazinyl]carbonyl]-4-fluorophenyl]methyl]-1(2H)-phthalazinonato]difluoroboron
CAS Number
1380359-84-1
PubChem ID
70697686
InChI Key
IGUTVNUEFKPBGK-UHFFFAOYSA-N
SMILES
CC1=CC(C)=[N+]2C1=CC3=CC=C(N3[B-]2(F)F)CCC(N4CCN(C(C5=CC(CC6=NNC(C7=CC=CC=C67)=O)=CC=C5F)=O)CC4)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.4 | 10 |
Preparing Stock Solutions
for PARPi-FL
The following data is based on the product molecular weight 640.46
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 15.61 mL | 78.07 mL | 156.14 mL |
| 0.5 mM | 3.12 mL | 15.61 mL | 31.23 mL |
| 1 mM | 1.56 mL | 7.81 mL | 15.61 mL |
| 5 mM | 0.31 mL | 1.56 mL | 3.12 mL |
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Background References
References are publications that support the biological activity of the product.
- Thurber Effect of small-molecule modification on single-cell pharmacokinetics of PARP inhibitors. Mol.Cancer Ther. 2014 PMID: 24552776
- Dubach Quantitating drug-target engagement in single cells in vitro and in vivo. Nat.Chem.Biol. 2017 PMID: 27918558
- Thurber Single-cell and subcellular pharmacokinetic imaging allows insight into drug action in vivo. Nat.Commun. 2013 PMID: 23422672
Product Specific Notices for PARPi-FL
For research use only
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