OGDA
Tocris Bioscience, part of Bio-Techne | Catalog # 7408
Green fluorescent D-amino acid; compatible with STED microscopy
Key Product Details
Description
Green fluorescent D-amino acid; compatible with STED microscopy
Alternative Names
OG fluorescent D-amino acid,OG FDAA
Product Description
OGDA is a green fluorescent D-amino acid. Suitable for labeling peptidoglycans in live bacteria. OGDA is efficiently incorporated into the peptidoglycans of gram-positive and some gram-negative bacteria. Compatible with STED microscopy, OGDA labelled bacteria can be imaged by super-resolution stimulated emission depletion (STED) at a resolution below 100 nm. Photo-stable. Excitation/emission λ ~501/526 nm.Optical Data
| Emission Color | Green |
| λabs | 501 nm |
| λem | 526 nm |
| Closest Laser line | 488 nm |
| Application | Super Resolution Microscopy (SRM), STED, Confocal Microscopy |
Spectra Viewer
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Product Specifications
Molecular Weight
498.39
Formula
C24H16F2N2O8
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(R)-2-Amino-3-(2',7'-difluoro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-6-carboxamido)propanoic acid
CAS Number
2758364-01-9
InChI Key
RFRUFDKIAPOSBS-MRXNPFEDSA-N
SMILES
O=C(NC[C@H](C(O)=O)N)C1=CC=C(C2=C1)C(OC32C4=C(OC5=C3C=C(C(O)=C5)F)C=C(O)C(F)=C4)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 49.84 | 100 |
Preparing Stock Solutions
for OGDA
The following data is based on the product molecular weight 498.39
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.01 mL | 10.03 mL | 20.06 mL |
| 5 mM | 0.40 mL | 2.01 mL | 4.01 mL |
| 10 mM | 0.20 mL | 1.00 mL | 2.01 mL |
| 50 mM | 0.04 mL | 0.20 mL | 0.40 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Söderström An OregonGreen488-labelled D-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy. Microbiology 2020 PMID: 33237852
Product Specific Notices for OGDA
For research use only
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