O-Propargyl-puromycin
Tocris Bioscience, part of Bio-Techne | Catalog # 7391
Puromycin analog for labeling newly synthesized proteins
Key Product Details
Description
Puromycin analog for labeling newly synthesized proteins
Product Description
O-Propargyl-puromycin (OP-puro) is a puromycin analog that is incorporated into nascent polypeptides. It is used for the labeling of newly synthesized proteins in cells and whole animals in vivo. O-Propargyl-puromycin can be click-conjugated to fluorescent azides for visualization by fluoresence microscopy. The product does not require methionine-free conditions. It can also be used in flow cytometry.Optical Data
| λem | 275 nm |
| Reactivity | Click |
| Application | Fluorescence Microscopy, Flow Cytometry |
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Product Specifications
Molecular Weight
495.54
Formula
C24H29N7O5
Storage
Store at -20°C
Purity
≥97% (HPLC)
Chemical Name
3'-[[(2S)-2-Amino-1-oxo-3-[4-(2-propyn-1-yloxy)phenyl]propyl]amino]-3'-deoxy-N,N-dimethyladenosine
CAS Number
1416561-90-4
PubChem ID
71576433
InChI Key
JXBIGWQNNSJLQK-IYRMOJGWSA-N
SMILES
O[C@@H]1[C@H](NC([C@@H](N)CC2=CC=C(OCC#C)C=C2)=O)[C@@H](CO)O[C@H]1N3C4=NC=NC(N(C)C)=C4N=C3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 49.55 | 100 |
Preparing Stock Solutions
for O-Propargyl-puromycin
The following data is based on the product molecular weight 495.54
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.02 mL | 10.09 mL | 20.18 mL |
| 5 mM | 0.40 mL | 2.02 mL | 4.04 mL |
| 10 mM | 0.20 mL | 1.01 mL | 2.02 mL |
| 50 mM | 0.04 mL | 0.20 mL | 0.40 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Morral Zonation of ribosomal DNA transcription defines a stem cell hierarchy in colorectal cancer. Cell Stem Cell 2020 PMID: 32396863
- Liu Imaging protein synthesis in cells and tissues with an alkyne analog of puromycin. Proc.Natl.Acad.Sci.USA 2012 PMID: 22160674
Product Specific Notices for O-Propargyl-puromycin
For research use only
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