Nile Red

Name: Nile Red
Brand: Tocris Bioscience
SKU: 7387

Tocris Bioscience, part of Bio-Techne | Catalog # 7387

COA / SDS

Fluorogenic lipid membrane dye. Used for: intracellular lipid droplets and neutral lipids staining, suitable for live cell imaging. Application: fluorescence microscopy

Key Product Details

Description:	Fluorogenic lipid membrane dye. Used for: intracellular lipid droplets and neutral lipids staining, suitable for live cell imaging. Application: fluorescence microscopy
Alternative Names:	Nile blue oxazone
Chemical Name:	9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one
Purity:	≥98% (HPLC)
Molecular Weight:	318.38

View Full Technical Details for Nile Red

Technical Data
Citations

Biological Activity for Nile Red

Key information: Nile red is a fluorogenic lipid membrane dye.

Used for: staining of intracellular lipid droplets and neutral lipids. It is suitable for long-term monitoring of lipid dynamics in living cells.

Application: fluorescence microscopy.

Properties and Photophysical Data: Nile red fluoresces in the hydrophobic environment of lipid membranes. Non-fluorescent in polar environments (e.g. water). Excitation and emission maxima (λ) are 552 nm and 636 nm in methanol, respectively.

Optical Data for Nile Red

Emission Color	Red
λ_abs	552 nm
λ_em	636 nm
Application	Fluorescence Microscopy, Monitoring of Lipid Dynamics in Living Cells

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for Nile Red

M. Wt	318.38
Formula	C₂₀H₁₈N₂O₂
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	7385-67-3
PubChem ID	65182
InChi Key	VOFUROIFQGPCGE-UHFFFAOYSA-N
Smiles	CCN(C1=CC2=C(N=C3C(O2)=CC(C4=CC=CC=C34)=O)C=C1)CC

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Nile Red

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	3.18	10

Preparing Stock Solutions for Nile Red

The following data is based on the product molecular weight 318.38.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
0.1 mM	31.41 mL	157.05 mL	314.09 mL
0.5 mM	6.28 mL	31.41 mL	62.82 mL
1 mM	3.14 mL	15.70 mL	31.41 mL
5 mM	0.63 mL	3.14 mL	6.28 mL

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References for Nile Red

References are publications that support the biological activity of the product.

Zhanghao High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. Nat.Commun. 2020 PMID: 33208737
Uribe-Etxebarria Wnt signaling reprograms metabolism in dental pulp stem cells. J.Cell Physiol. 2019 PMID: 30549037
Maes A rapid-screening approach to detect and quantify microplastics based on fluorescent tagging with Nile red. Sci.Rep 2017 PMID: 28300146