Mpro Tracer
Tocris Bioscience, part of Bio-Techne | Catalog # 8126
SARS-CoV-2 main protease fluorescent ligand; TR-FRET acceptor
Key Product Details
Description
SARS-CoV-2 main protease fluorescent ligand; TR-FRET acceptor
Product Description
Mpro Tracer is a high affinity (Kd = 51 nM) non-covalent SARS-CoV-2 main protease fluorescent ligand. Mpro Tracer can be used in fluorescent polarisation (FP) assay for inhibitor screening/characterisation. It can be used as a FRET acceptor in time-resolved fluorescence resonance energy-transfer (TR-FRET) experiments. Excitation and emission maxima (λ) are 492 nm and 518 nm.Scientific Data Images for Mpro Tracer
Fluorescence polarisation assay to determine the inhibitory activity of nirmatrelvir on purified SARS-CoV-2 Main protease protein (Mpro)
Mpro Tracer and purified Mpro were used at a concentration of 1 μM. A dose response over a range of inhibitor concentrations were measured. Assays were performed in 100 μL wells and left for 1 hour at room temperature before FP measurements were taken. Curve was plotted and fit using graphpad to obtain an IC50 value of 0.74 μM under the assay conditions. Data provided by the Hilgenfeld lab, Lubeck University.
3CLpro-C10778AA-FITC tracer HTRF assay
The Kd of Mpro tracer was measured by varying the tracer concentration against a range of recombinant His6-tagged Mpro concentrations using an HTRF (TR-FRET) assay. MAb Anti-6HIS-Tb GOLD was used as the FRET donor and Mpro Tracer as the FRET acceptor (abs/em = 492nm/518nm). HTRF ratio (520/495) was determined using as Envision plate reader, measurement were recorded after 90 minutes. The Kd of Mpro Tracer was determined to be 51 nM.Optical Data
| Emission Color | Green |
| λabs | 492 nm |
| λem | 518 nm |
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Product Specifications
Molecular Weight
909.41
Storage
Store at -20°C
Purity
≥95% (HPLC)
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 9.09 | 10 |
Preparing Stock Solutions
for Mpro Tracer
The following data is based on the product molecular weight 909.41
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 11.00 mL | 54.98 mL | 109.96 mL |
| 0.5 mM | 2.20 mL | 11.00 mL | 21.99 mL |
| 1 mM | 1.10 mL | 5.50 mL | 11.00 mL |
| 5 mM | 0.22 mL | 1.10 mL | 2.20 mL |
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Product Specific Notices for Mpro Tracer
For research use only
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