MitoPY1
Tocris Bioscience, part of Bio-Techne | Catalog # 4428
Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy
Key Product Details
Description
Fluorescent mitochondrial hydrogen peroxide indicator. Used for: live cell imaging. Application: confocal microscopy
Product Description
Key information: mitochondria peroxy yellow 1 (MitoPY1) is a fluorescent probe for imaging hydrogen peroxide (H2O2) in mitochondria of living cells.Used for: imaging hydrogen peroxide (H2O2) in mitochondria of living cells. Can also detect local increases in H2O2 in an in vitro model of Parkinson's Disease.
Application: confocal microscopy.
Properties and Photophysical Data: selective for H2O2 over superoxide, nitric oxide and hydroxyl radical.
Excitation and emission maxima (λ) are 510 nm and 530 nm, respectively, quantum yield = 0.405.
Licensing Information
Sold under license from the Regents of the University of CaliforniaOptical Data
| λabs | 510 nm |
| λem | 530 nm |
| Quantum Yield (φ) | 0.405 |
| Application | Confocal Imaging |
Spectra Viewer
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Product Specifications
Molecular Weight
954.68
Formula
C52H53BIN2O5P
Storage
Store at -20°C
Chemical Name
[4-[4-[3-Oxo-6'-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3'-yl]-1-piperazinyl]butyl]triphenyl-phosphonium iodide
CAS Number
1041634-69-8
PubChem ID
90488942
InChI Key
JPCCULDYUUMFAD-UHFFFAOYSA-M
SMILES
O=C(O4)C5=C(C=CC=C5)C24C1=CC=C(N6CCN(CCCC[P+](C9=CC=CC=C9)(C%10=CC=CC=C%10)C8=CC=CC=C8)CC6)C=C1OC3=C2C=CC(B7OC(C)(C)C(C)(C)O7)=C3.[I-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 95.47 | 100 |
Preparing Stock Solutions
for MitoPY1
The following data is based on the product molecular weight 954.68
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.05 mL | 5.24 mL | 10.47 mL |
| 5 mM | 0.21 mL | 1.05 mL | 2.09 mL |
| 10 mM | 0.10 mL | 0.52 mL | 1.05 mL |
| 50 mM | 0.02 mL | 0.10 mL | 0.21 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Zhao Lighting up H2O2: the molecule that is a "necessary evil" in the cell. Angew.Chem.Int.Ed.Engl. 2009 PMID: 19165849
- Guo Two-photon fluorescence imaging of intracellular hydrogen peroxide with chemoselective fluorescent probes. J.Biomed.Opt. 2013 PMID: 24084856
- Dickinson and Chang A targetable fluorescent probe for imaging hydrogen peroxide in the mitochondria of living cells. J.Am.Chem.Soc. 2008 PMID: 18605728
Product Specific Notices for MitoPY1
For research use only
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