Mito-HE
Tocris Bioscience, part of Bio-Techne | Catalog # 7641
Red-fluorescent mitochondrial superoxide indicator. Used for: live cell imaging. Application: confocal microscopy, flow cytometry
Key Product Details
Description
Red-fluorescent mitochondrial superoxide indicator. Used for: live cell imaging. Application: confocal microscopy, flow cytometry
Product Description
Key information: Mito-HE is a red fluorescent mitochondrial superoxide indicator in live cells.Used for: live cell imaging, mitochondrial superoxide detection in Parkinson's and Alzheimer's disease in vitro models.
Application: confocal imaging, flow cytometry.
Properties and Photophysical Data: Selective for mitochondrial superoxides over other reactive oxygen and nitrogen species. Excitation and emission maxima (λ) are 510 nm and 580 nm, respectively; extinction coefficient = 117,000 M-1cm-1.
It is recommended to prepare stock solutions in DMSO.
Optical Data
| Emission Color | Red |
| λabs | 510 nm |
| λem | 580 nm |
| Extinction Coefficient (ε) | 11700 M-1cm-1 |
| Cell Permeable | Yes |
| Application | Confocal Imaging, Flow Cytometry |
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Product Specifications
Molecular Weight
759.72
Formula
C43H43IN3P
Storage
Store at -20°C
Purity
≥80% (HPLC)
Chemical Name
[6-(3,8-Diamino-6-phenyl-5(6H)-phenanthridinyl)hexyl]triphenylphosphonium iodide
CAS Number
1003197-00-9
PubChem ID
160231797
InChI Key
NMKOJFMSKHLXRV-UHFFFAOYSA-M
SMILES
NC1=CC=C2C(C(C3=CC=CC=C3)N(C4=CC(N)=CC=C24)CCCCCC[P+](C5=CC=CC=C5)(C6=CC=CC=C6)C7=CC=CC=C7)=C1.[I-]
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Chen Trilobatin protects against Aβ 25-35-induced hippocampal HT22 cells apoptosis through mediating ROS/p38/caspase 3-dependent pathway. Front.Pharmacol. 2020 PMID: 32508629
- Chung Parkin and PINK1 patient iPSC-derived midbrain dopamine neurons exhibit mitochondrial dysfunction and α-synuclein accumulation. Stem Cell Rep. 2016 PMID: 27641647
- Robinson Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc.Natl.Acad.Sci.U.S.A. 2006 PMID: 17015830
- Mukhopadhyay Simultaneous detection of apoptosis and mitochondrial superoxide production in live cells by flow cytometry and confocal microscopy. Nat.Protoc. 2007 PMID: 17853886
- Mukhopadhyay Simple quantitative detection of mitochondrial superoxide production in live cells. Biochem.Biophys.Res.Commun. 2007 PMID: 17475217
- Renaudin BRCA2 deficiency reveals that oxidative stress impairs RNaseH1 function to cripple mitochondrial DNA maintenance. Cell Rep. 2021 PMID: 34348152
- Rhee Modeling secondary iron overload cardiomyopathy with human induced pluripotent stem cell-derived cardiomyocytes. Cell Rep. 2020 PMID: 32668256
Product Specific Notices for Mito-HE
For research use only
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