K 114

Name: K 114
Brand: Tocris Bioscience
SKU: 3144

Tocris Bioscience, part of Bio-Techne | Catalog # 3144

COA / SDS

Amyloid fibril-specific fluorescent probe. Used for: amyloid fibril detection. Application: fluorescence microscopy, confocal microscopy

Key Product Details

Description:	Amyloid fibril-specific fluorescent probe. Used for: amyloid fibril detection. Application: fluorescence microscopy, confocal microscopy
Chemical Name:	4,4'-[(2-Bromo-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
Purity:	≥98% (HPLC)
Molecular Weight:	393.27

View Full Technical Details for K 114

Technical Data
Citations

Biological Activity for K 114

Key information: K 114 is a potent amyloid fibril-specific fluorescent probe (EC₅₀ = 20 - 30 nM).

Used for: amyloid fibril detection.

Application: fluorescence microscopy, confocal microscopy.

Properties and Photophysical Data: K 114 exhibits minimal fluorescence in aqueous buffers and fluoresces brightly in the presence of Aβ (1-40), α-synuclein and tau in situ. K 114 fluorescence is ph-dependent. Excitation and emission maxima (λ) are 370nm and 450 nm, respectively, at pH 8.5; excitation and emission maxima (λ) are 395nm and 520 nm, respectively, at pH 10.5.

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Technical Data for K 114

M. Wt	393.27
Formula	C₂₂H₁₇BrO₂
Storage	Store at +4°C
Purity	≥98% (HPLC)
CAS Number	872201-12-2
PubChem ID	44828481
InChi Key	OXPHQQMZTXMEGO-RJTULKDBSA-N
Smiles	OC(C=C3)=CC=C3/C=C/C1=CC(Br)=C(/C=C/C2=CC=C(O)C=C2)C=C1

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for K 114

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
	DMSO	39.33	100
	ethanol	19.66	50

Preparing Stock Solutions for K 114

The following data is based on the product molecular weight 393.27.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.54 mL	12.71 mL	25.43 mL
5 mM	0.51 mL	2.54 mL	5.09 mL
10 mM	0.25 mL	1.27 mL	2.54 mL
50 mM	0.05 mL	0.25 mL	0.51 mL

Molarity Calculator

Calculate the mass, volume, or concentration required for a solution.

Mass

Concentration

Volume

M. Wt*

g/mol

*When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and CoA (available online).

Dilution Calculator

Calculate the dilution required to prepare a stock solution.

Concentration 1 (C1)

Volume 1 (V1)

Concentration 2 (C2)

Volume 2 (V2)

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

References for K 114

References are publications that support the biological activity of the product.

LeVine Mechanism of Aβ(1-40) fibril-induced fluorescence of (trans-trans)-1-bromo-2,5-bis(4-hydroxystyryl)benene (K114). Biochemistry 2005 PMID: 16313197
Stepanchuk Complex photophysical properties of K114 make for a versatile fluorescent probe for amyloid detection. ACS Chem.Neurosci. 2021 PMID: 33705095
Crystal A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114. J.Neurochem. 2003 PMID: 12950445