K 114
Tocris Bioscience, part of Bio-Techne | Catalog # 3144
Amyloid fibril-specific fluorescent probe. Used for: amyloid fibril detection. Application: fluorescence microscopy, confocal microscopy
Key Product Details
Description
Amyloid fibril-specific fluorescent probe. Used for: amyloid fibril detection. Application: fluorescence microscopy, confocal microscopy
Product Description
Key information: K 114 is a potent amyloid fibril-specific fluorescent probe (EC50 = 20 - 30 nM).Used for: amyloid fibril detection.
Application: fluorescence microscopy, confocal microscopy.
Properties and Photophysical Data: K 114 exhibits minimal fluorescence in aqueous buffers and fluoresces brightly in the presence of Aβ (1-40), α-synuclein and tau in situ. K 114 fluorescence is ph-dependent. Excitation and emission maxima (λ) are 370nm and 450 nm, respectively, at pH 8.5; excitation and emission maxima (λ) are 395nm and 520 nm, respectively, at pH 10.5.
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Product Specifications
Molecular Weight
393.27
Formula
C22H17BrO2
Storage
Store at +4°C
Purity
≥98% (HPLC)
Chemical Name
4,4'-[(2-Bromo-1,4-phenylene)di-(1E)-2,1-ethenediyl]bisphenol
CAS Number
872201-12-2
PubChem ID
44828481
InChI Key
OXPHQQMZTXMEGO-RJTULKDBSA-N
SMILES
OC(C=C3)=CC=C3/C=C/C1=CC(Br)=C(/C=C/C2=CC=C(O)C=C2)C=C1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 39.33 | 100 | |
| Ethanol | 19.66 | 50 |
Preparing Stock Solutions
for K 114
The following data is based on the product molecular weight 393.27
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.54 mL | 12.71 mL | 25.43 mL |
| 5 mM | 0.51 mL | 2.54 mL | 5.09 mL |
| 10 mM | 0.25 mL | 1.27 mL | 2.54 mL |
| 50 mM | 0.05 mL | 0.25 mL | 0.51 mL |
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Background References
References are publications that support the biological activity of the product.
- LeVine Mechanism of Aβ(1-40) fibril-induced fluorescence of (trans-trans)-1-bromo-2,5-bis(4-hydroxystyryl)benene (K114). Biochemistry 2005 PMID: 16313197
- Stepanchuk Complex photophysical properties of K114 make for a versatile fluorescent probe for amyloid detection. ACS Chem.Neurosci. 2021 PMID: 33705095
- Crystal A comparison of amyloid fibrillogenesis using the novel fluorescent compound K114. J.Neurochem. 2003 PMID: 12950445
Product Specific Notices for K 114
For research use only
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