Janelia Fluor® 635, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 6419
Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging
Key Product Details
Description
Red dye (Reactive group: NHS ester). Application: flow cytometry, confocal microscopy, super resolution microscopy. Suitable for live cell imaging
Alternative Names
JF 635,NHS
Product Description
Key Information: Janelia Fluor® 635, NHS ester is a red fluorogenic fluorescent dye; supplied with an NHS ester reactive group for the labeling of primary amines. Suitable for live cell imaging.Application: Suitable for flow cytometry, confocal microscopy, super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells). Cell permeable.
Properties and Photophysical Data: NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Excitation and emission maxima (λ) are 635 nm and 652 nm, respectively; quantum yield = 0.56; extinction coefficient = 167,000 M-1cm-1 (measured in ethanol or TFE plus 0.1% TFA); A280 correction factor = 0.0524; lactone-zwitterion equilibrium constant (KL-Z) <0.0001. Exhibits low nonspecific background staining.
Please see the protocol for further information on protein/antibody labeling and conjugation.
To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA.
We also offer custom conjugation antibody services.
HaloTag® is a registered trademark of Promega Corporation, and SNAP-tag® is a registered trademark of New England BioLabs, Inc.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
This product has been recently renamed. The previous name for this product was Janelia Fluor® 635, SE.
Scientific Data Images for Janelia Fluor® 635, NHS ester
Applications on mitochondria for Janelia Fluor® 635, NHS ester.
Application of Janelia Fluor® 635, NHS ester dye on mitochondria; Left: Mitochondria were labeled with anti-TOM20 primary antibodies. The secondary antibodies were conjugated to JF 635 dye. Maximum intensity projection of confocal z-stack, recorded on an Olympus FV1200 confocal microscope. Right: Enlarged portion of left-hand image. Images kindly provided by Dr Kirstin Elgass, Monash University.Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Red |
| Brightness | |
| λabs | 635 nm |
| λem | 652 nm |
| Extinction Coefficient (ε) | 167,000 M-1cm-1 |
| Quantum Yield (φ) | 0.56 |
| Closest Laser line | 635 nm |
| Reactive Group | NHS ester |
| Reactivity | Primary amines |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.0524 |
| Application | Confocal Microscopy, Super Resolution Microscopy, Flow Cytometry |
| Alternative For | Alexa Fluor® 633, Atto 633, CF633 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications
Molecular Weight
629.69
Formula
C33H29F2N3O6Si
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
1-[10-[2-Carboxy-5-[[(2,5-dioxo-1-pyrrolidinyl)oxy]carbonyl]phenyl]-9,9-dimethyl-7-[1-(3-fluoroazetidinyl)]-9-silaanthracen-2(9H)-ylidene]-3-fluoroazetidinium, inner salt
PubChem ID
132470914
InChI Key
YVKNNZJUJGADIJ-UHFFFAOYSA-N
SMILES
O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)C4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)C\7)=C2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 62.97 | 100 |
Preparing Stock Solutions
for Janelia Fluor® 635, NHS ester
The following data is based on the product molecular weight 629.69
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.59 mL | 7.94 mL | 15.88 mL |
| 5 mM | 0.32 mL | 1.59 mL | 3.18 mL |
| 10 mM | 0.16 mL | 0.79 mL | 1.59 mL |
| 50 mM | 0.03 mL | 0.16 mL | 0.32 mL |
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Background References
References are publications that support the biological activity of the product.
- Zheng Rational design of fluorogenic and spontaneously blinking labels for super-resolution imaging. ACS Cent.Sci. 2019 PMID: 31572787
- Grimm A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 2017 PMID: 28869757
Product Specific Notices for Janelia Fluor® 635, NHS ester
For research use only
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