Janelia Fluor® 549, Tetrazine
Tocris Bioscience, part of Bio-Techne | Catalog # 6502
Yellow dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Key Product Details
Description
Yellow dye (Reactive group: tetrazine). Application: confocal microscopy, super resolution microscopy including dSTORM & STED. Suitable for live cell imaging
Product Description
Key Information: Janelia Fluor® 549, Tetrazine is a yellow fluorescent dye; supplied with a tetrazine reactive handle for copper-free click chemistry. Suitable for live-cell imaging.Application: Suitable for confocal microscopy and super resolution microscopy (SRM) techniques including dSTORM (in both live and fixed cells) and STED. Janelia Fluor® 549, tetrazine is cell permeable. It can be coupled directly to a protein of interest using bioorthogonal chemistry and unnatural amino acid technology and has been used in this way to label living primary neurons.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 549 nm and 571 nm, respectively; quantum yield = 0.88; extinction coefficient = 101,000 M-1cm-1; A280 correction factor = 0.169.
Janelia Fluor® is a registered trademark of Howard Hughes Medical Institute.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| Brightness | |
| λabs | 549 nm |
| λem | 571 nm |
| Extinction Coefficient (ε) | 101,000 M-1cm-1 |
| Quantum Yield (φ) | 0.88 |
| Closest Laser line | 532 nm |
| Reactive Group | Tetrazine |
| Reactivity | Copper-free click |
| Cell Permeable | Yes |
| Correction Factor 280 | 0.169 |
| Application | Confocal Microscopy, dSTORM, STED, Live-cell Imaging |
| Alternative For | Alexa Fluor® 546, Alexa Fluor® 555, BDY TMR-X, Atto 550, CF 555, TAMRA, Cyanine 3 |
Trademarks and registered trademarks are the property of their respective owners.
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Product Specifications
Molecular Weight
623.66
Formula
C36H29N7O4
Storage
Store at -20°C
Chemical Name
3,6-Di-1-azetidinyl-9-[[4-[(1,2,4,5-tetrazin-3-yl)benzyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
SMILES
O=C(NCC1=CC=C(C2=NN=CN=N2)C=C1)C3=CC=C(C([O-])=O)C(C(C4=CC=C(N5CCC5)C=C4O6)=C(C=C/7)C6=CC7=[N+]8CCC\8)=C3
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.24 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 549, Tetrazine
The following data is based on the product molecular weight 623.66
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 16.03 mL | 80.17 mL | 160.34 mL |
| 0.5 mM | 3.21 mL | 16.03 mL | 32.07 mL |
| 1 mM | 1.60 mL | 8.02 mL | 16.03 mL |
| 5 mM | 0.32 mL | 1.60 mL | 3.21 mL |
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Background References
References are publications that support the biological activity of the product.
- Peng and Hang Site-specific bioorthogonal labeling for fluorescence imaging of intracellular proteins in living cells. J.Am.Chem.Soc. 2016 PMID: 27768298
- Arsic Minimal genetically encoded tags for fluorescent protein labeling in living neurons. Nat.Commun. 2022 PMID: 35031604
Product Specific Notices for Janelia Fluor® 549, Tetrazine
For research use only
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