Janelia Fluor® 525 ADA New
Tocris Bioscience, part of Bio-Techne | Catalog # 8962
Yellow JF-based fluorescent dye for staining live Staphylococcus aureus bacteria
Key Product Details
Description
Yellow JF-based fluorescent dye for staining live Staphylococcus aureus bacteria
Product Description
Key information: Janelia Fluor® 525 ADA is a yellow fluorescent dye suitable for bacterial imaging applications, consisting of a bright Janelia Fluor® fluorophore bearing the unnatural amino acid D-Alanine. Incorporation of fluorescent D-amino acids in gram negative and positive bacteria can selectively label bacterial membranes, resulting in bright fluorescence around the bacteria and minimal or no signal detected inside.Used for: selectively staining Staphylococcus aureus.
Application: confocal microscopy and flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 525 nm and 549 nm, respectively; quantum yield = 0.91; extinction coefficient = 122,000 M-1cm-1; lactone-zwitterion equilibrium constant (KL-Z) = 0.068.
Licensing Information
Sold under license from the Howard Hughes Medical Institute, Janelia Research CampusOptical Data
| Emission Color | Yellow |
| λabs | 525 nm |
| λem | 549 nm |
| Extinction Coefficient (ε) | 122,000 M-1cm-1 |
| Quantum Yield (φ) | 0.91 |
| Correction Factor 280 | 0.185 |
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Product Specifications
Molecular Weight
840.58
Formula
C30H24F4N4O6.2CF3CO2H
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(R)-4-((2-Amino-2-carboxyethyl)carbamoyl)-2-(3-(3,3-difluoroazetidin-1-ium-1-ylidene)-6-(3,3-difluoroazetidin-1-yl)-3H-xanthen-9-yl)benzoate ditrifluoroacetate
InChI Key
RRHAUQCSJYRQEK-GJICFQLNSA-N
SMILES
O=C(NC[C@@H](N)C(O)=O)C1=CC=C(C([O-])=O)C(C(C2=C(O3)C=C(N4CC(F)(F)C4)C=C2)=C5C3=C/C(C=C5)=[N+]6CC(F)(F)C\6)=C1.OC(C(F)(F)F)=O.OC(C(F)(F)F)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 8.41 | 10 |
Preparing Stock Solutions
for Janelia Fluor® 525 ADA
The following data is based on the product molecular weight 840.58
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 11.90 mL | 59.48 mL | 118.97 mL |
| 0.5 mM | 2.38 mL | 11.90 mL | 23.79 mL |
| 1 mM | 1.19 mL | 5.95 mL | 11.90 mL |
| 5 mM | 0.24 mL | 1.19 mL | 2.38 mL |
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Product Specific Notices for Janelia Fluor® 525 ADA
For research use only
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