IRDye® 680RD, NHS ester
Tocris Bioscience, part of Bio-Techne | Catalog # 8878
Near-infared dye (reactive group NHS ester)
Key Product Details
Description
Near-infared dye (reactive group NHS ester)
Product Description
Key information: IRDye® 680RD, NHS ester is a near-infrared (NIR) fluorescent dye, supplied with an NHS ester reactive group for the labeling of primary amines.Application: Fluorescence microscopy.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 680 nm and 694 nm, respectively.
Please see the protocol for further information on protein/antibody labeling and conjugation.
NOTE: The choice of solvent for the dye is dependent on the stability of the protein in DMSO. Since a small amount of dye is needed to label large (i.e. >45 kDA) proteins, the amount of DMSO in the reaction is negligible and should not affect protein stability. However, since a proportionally larger amount of dye is required to achieve adequate labeling for a small protein (i.e. <45 kDA), the level of DMSO in the reaction is more likely to affect the protein stability. Dye solubilized in water should be discarded after one use. Dye prepared in DMSO is stable for up to two weeks when stored at -20°C and protected from light and moisture.
It is recommended to dissolve dye in water or DMSO (10 - 20 mg/mL)
IRDye® is a registered trademark of Li-Cor and is used under license.
Optical Data
| λabs | 680 nm |
| λem | 694 nm |
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Product Specifications
Molecular Weight
1003.46
Formula
C43H45ClN4Na2O13S3
Storage
Store at -20°C
Purity
≥70% (HPLC)
Chemical Name
Sodium (Z)-2-((2Z,4E)-5-(5-Chloro-3,3-dimethyl-7-(3-sulfonatopropyl)-3H-pyrrolo[2,3-b]pyridin-7-ium-2-yl)-3-(3-(3-((2,5-dioxopyrrolidin-1-yl)oxy)-3-oxopropyl)phenyl)penta-2,4-dien-1-ylidene)-3,3-dimethyl-1-(3-sulfonatopropyl)indoline-5-sulfonate
CAS Number
1373919-87-9
InChI Key
JNFTWCVWRDPJNM-UHFFFAOYSA-L
SMILES
O=S(C1=CC(C/2(C)C)=C(C=C1)N(CCCS(=O)(O[Na])=O)C2=C/C=C(C3=CC(CCC(ON4C(CCC4=O)=O)=O)=CC=C3)/C=C/C5=NC6=[N+](CCCS([O-])(=O)=O)C=C(Cl)C=C6C5(C)C)(O[Na])=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- Al-Hawat Development of a polymersome blood ammonia assay coupled with a portable near-infrared fluorometer. ACS Bio.Med.Chem.Au. 2024 PMID: 39431266
Product Specific Notices for IRDye® 680RD, NHS ester
For research use only
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