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H2DCFDA

Tocris Bioscience, part of Bio-Techne | Catalog # 5935

Fluorescent ROS indicator; cell permeable
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5935/100

Key Product Details

Description

Fluorescent ROS indicator; cell permeable

Product Description

H2DCFDA is a fluorescent ROS indicator; becomes fluorescent when oxidized. Excitation wavelength ~490 nm, emission ~520 nm. Cell permeable.

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Product Specifications

Molecular Weight

487.29

Formula

C24H16Cl2O7

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

2-[3,6-Bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]benzoic acid

CAS Number

4091-99-0

PubChem ID

77718

InChI Key

PXEZTIWVRVSYOK-UHFFFAOYSA-N

SMILES

ClC1=CC(C(C2=CC=CC=C2C(O)=O)C(C=C(Cl)C(OC(C)=O)=C3)=C3O4)=C4C=C1OC(C)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Solubility

Solvent Max Conc. mg/mL Max Conc. mM
Solubility
DMSO 48.73 100
Ethanol 4.87 10

Preparing Stock Solutions for H2DCFDA

The following data is based on the product molecular weight 487.29

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass 1 mg 5 mg 10 mg
1 mM 2.05 mL 10.26 mL 20.52 mL
5 mM 0.41 mL 2.05 mL 4.10 mL
10 mM 0.21 mL 1.03 mL 2.05 mL
50 mM 0.04 mL 0.21 mL 0.41 mL

Calculators

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Reconstitution Calculator

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Background References

References are publications that support the biological activity of the product.

Product Documents for H2DCFDA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for H2DCFDA

For research use only

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