Fluorescein Tyramide
Tocris Bioscience, part of Bio-Techne | Catalog # 6456
Green fluorescent reagent widely used for signal amplification in IHC and FISH
Key Product Details
Description
Green fluorescent reagent widely used for signal amplification in IHC and FISH
Product Description
Fluorescein Tyramide is a green fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide to adjacent tyrosines to enhance fluorescent signal.Optical Data
| Emission Color | Green |
| λabs | 494 nm |
| λem | 517 nm |
| Extinction Coefficient (ε) | 70000 M-1cm-1 |
| Closest Laser line | 488 nm |
| Application | Tyramide Signal Amplification (TSA) in IHC, ICC, ISH, FISH |
Spectra Viewer
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Product Specifications
Molecular Weight
495.48
Formula
C29H21NO7
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
3',6'-Dihydroxy-N-(4-hydroxyphenylethyl)-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5(6)-carboxamide
CAS Number
210236-90-1
InChI Key
MJQMGAFVBOOBIB-UHFFFAOYSA-N
SMILES
OC1=CC2=C(C3(C4=CC=C(C=C4O2)O)C5=CC=C(C(NCCC6=CC=C(C=C6)O)=O)C=C5C(O3)=O)C=C1.OC7=CC8=C(C9(C%10=CC=C(C=C%10O8)O)C%11=CC(C(NCCC%12=CC=C(C=C%12)O)=O)=CC=C%11C(O9)=O)C=C7
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.89 | 24 |
Preparing Stock Solutions
for Fluorescein Tyramide
The following data is based on the product molecular weight 495.48
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.24 mM | 8.41 mL | 42.05 mL | 84.09 mL |
| 1.2 mM | 1.68 mL | 8.41 mL | 16.82 mL |
| 2.4 mM | 0.84 mL | 4.20 mL | 8.41 mL |
| 12 mM | 0.17 mL | 0.84 mL | 1.68 mL |
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Background References
References are publications that support the biological activity of the product.
- Bobrow Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays. J.Immunol.Methods 1989 PMID: 2558138
- van Gijlswijk Fluorochrome-labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J.Histochem.Cytochem. 1997 PMID: 9071319
- Schriml Tyramide signal amplification (TSA)-FISH applied to mapping PCR-labeled probes less than 1 kb in size. Biotechniques 1999 PMID: 10489619
- Hopman Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification. J.Histochem.Cytochem. 1998 PMID: 9603790
- Karkmann Enzymatic signal amplification for sensitive detection of intracellular antigens by flow cytometry. J.Immunol.Methods 1999 PMID: 10594358
Product Specific Notices for Fluorescein Tyramide
For research use only
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