Fluo-4 AM

Tocris Bioscience, part of Bio-Techne | Catalog # 6255

Cell-permeable, fluorescent Ca2+ indicator
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Key Product Details

Description

Cell-permeable, fluorescent Ca2+ indicator

View all Calcium Signaling Probes and Ca2+ Indicator Dyes Fluorescent Probes and Dyes »

Product Description

Fluo-4 AM is a cell-permeable, fluorescent Ca2+ indicator (Kd Ca2+ = 345 nM). Displays no resting signal and 100-fold increase in emission intensity upon Ca2+ binding. Excitation/emission λ 494/506 nm.

It is recommended to prepare stock solutions in DMSO.

Optical Data

Emission Color Green
λabs 494 nm
λem 506 nm
Closest Laser line 488 nm
Cell Permeable Yes
Application Fluorescence Microscopy, Flow Cytometry, Fluorometric Microplate Reading Assays

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Product Specifications

Molecular Weight

1096.95

Formula

C51H50F2N2O23

Storage

Store at -20°C

Purity

≥95% (HPLC)

Chemical Name

N-[4-[6-[(Acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]glycine (acetyloxy)methyl ester

CAS Number

273221-67-3

PubChem ID

4060965

InChI Key

QOMNQGZXFYNBNG-UHFFFAOYSA-N

SMILES

O=C(C(F)=C1)C=C2C1=C(C3=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C3)C5=CC(F)=C(OCOC(C)=O)C=C5O2

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

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Background References

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Product Documents for Fluo-4 AM

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fluo-4 AM

For research use only

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