Cy7ADA New
Tocris Bioscience, part of Bio-Techne | Catalog # 8901
Near-infrared Cy7-based fluorescent dye for live gram positive and negative bacteria imaging in vitro and in vivo
Key Product Details
Description
Near-infrared Cy7-based fluorescent dye for live gram positive and negative bacteria imaging in vitro and in vivo
Product Description
Key information: Cy7ADA is a near-infrared fluorescent D-amino acid (FDAA) probe suitable for bacterial imaging applications, consisting of a Cyanine 7 fluorophore bearing the unnatural amino acid D-Alanine. Incorporation of fluorescent D-amino acids in gram negative and positive bacteria can selectively label bacterial membranes, resulting in bright fluorescence around the bacteria and minimal or no signal detected inside.Used for: in situ labeling and imaging of gram positive (S. aureus, E. faecalis and S. sanguinis) and gram negative (E. coli) bacteria in vitro and selective staining of S. aureus in mice.
Application: confocal microscopy, intravital confocal fluorescence microscopy, flow cytometry.
Properties and Photophysical Data: Excitation and emission maxima (λ) are 756 nm and 779 nm, respectively.
Optical Data
| Emission Color | Near-IR |
| λabs | 756 nm |
| λem | 779 nm |
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Product Specifications
Molecular Weight
768.94
Formula
C38H48N4O9S2
Storage
Store at -20°C
Purity
≥95% (HPLC)
Chemical Name
(R)-2-Ammonio-3-(6-(2-((1E,3E,5E)-7-((E)-1-ethyl-3,3-dimethyl-5-sulfoindolin-2-ylidene)hepta-1,3,5-trien-1-yl)-3,3-dimethyl-5-sulfonato-3H-indol-1-ium-1-yl)hexanamido)propanoate
InChI Key
ILIGHKLJGHKXMU-SSEXGKCCSA-N
SMILES
CC(/C1=C\C=C\C=C\C=C\C2=[N+](CCCCCC(NC[C@H](C([O-])=O)[NH3+])=O)C3=C(C=C(C=C3)S(=O)([O-])=O)C2(C)C)(C4=CC(S(=O)(O)=O)=CC=C4N1CC)C
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.69 | 10 |
Preparing Stock Solutions
for Cy7ADA
The following data is based on the product molecular weight 768.94
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 0.1 mM | 13.00 mL | 65.02 mL | 130.05 mL |
| 0.5 mM | 2.60 mL | 13.00 mL | 26.01 mL |
| 1 mM | 1.30 mL | 6.50 mL | 13.00 mL |
| 5 mM | 0.26 mL | 1.30 mL | 2.60 mL |
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Background References
References are publications that support the biological activity of the product.
- Li In vivo labeling and intravital imaging of bacterial infection using a near-infrared fluorescent D-amino acid probe. ChemBioChem 2024 PMID: 38715148
Product Specific Notices for Cy7ADA
For research use only
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