ChromaLIVE™ Non-Toxic Dye
Tocris Bioscience, part of Bio-Techne | Catalog # 8934
Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells
Key Product Details
Description
Ready-to-use fluorescent probe for long term high content phenotypic screening of live cells
Product Description
ChromaLIVE™ Non-Toxic Dye is a ready-to-use fluorescent probe for long term high content phenotypic screening of live-cells. Optimised for live cell painting and morphological profiling in 2D monolayers, 3D spheroids or organoids, the dye provides multi organelle read out for unbiased classification of cell states such as proliferation, stress responses, autophagy, apoptosis or other phenotypic transitions. Excitation and emission maxima (λ) are 488 nM and 561 nM, respectively. ChromaLIVE™ Non-Toxic Dye is provided as 10 μL of 1000x concentrate in DMSO and is compatible with high-throughput and high-content screening workflows, including automated 96-, 384-, and 1536-well plate formats.Please refer to this protocol for further information on how to use this product.
ChromaLIVE™ is a trademark of Saguaro Biosciences.
Scientific Data Images for ChromaLIVE™ Non-Toxic Dye
ChromaLIVE™ Non-Toxic Dye Application.
High-content imaging application of HeLa cells treated with staurosporine for 20 hours, then stained with ChromaLIVE™ Non-Toxic Dye and Hoechst 33342. Image acquired on the Yokogawa CQ1.
ChromaLIVE™ Non-Toxic Dye Application.
High-content imaging of live MCF-7 cells stained with ChromaLIVE™ Non-Toxic Dye, imaged at 561–600 nm and 488–700 nm. Demonstrates live-cell compatibility and bright cytoplasmic signal.
ChromaLIVE™ Non-Toxic Dye Application.
High-content imaging of HEK293 spheroids stained with ChromaLIVE™ Non-Toxic Dye and imaged using the Yokogawa CQ3000. Six-tile panel displays treatment conditions left to right: DMSO, rapamycin (50 µM), and brefeldin A (10 µM); timepoints top to bottom: 24 h and 48 h.Licensing Information
Sold under license from Saguaro BiosciencesOptical Data
| λabs | 488 nm |
| λem | 561 nm |
| Cell Permeable | Yes |
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Product Specifications
Storage
Store at -20°C
Purity
≥90% (HPLC)
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble in DMSO (supplied pre-dissolved in DMSO, 1000x) |
Calculators
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Cottet Live cell painting: new nontoxic dye to probe cell physiology in high content screening. SLAS Discov. 2023 PMID: 39492484
- Sivagurunathan Alternate dyes for image-based profiling assays. SLAS Discov. 2025 PMID: 40885286
Product Specific Notices for ChromaLIVE™ Non-Toxic Dye
For research use only
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