Calcein AM
Tocris Bioscience, part of Bio-Techne | Catalog # 5119
Cell permeable non-fluorescent compound; green-fluorescent in living cells once hydrolyzed. Used for: cell tracing and cell viability monitoring. Application: fluorescent microscopy and flow cytometry
Key Product Details
Description
Cell permeable non-fluorescent compound; green-fluorescent in living cells once hydrolyzed. Used for: cell tracing and cell viability monitoring. Application: fluorescent microscopy and flow cytometry
Product Description
Key information: Calcein AM is a cell permeable non-fluorescent compound, that becomes green fluorescent once hydrolyzed in live cells.Used for: cell tracing, monitoring cell viability, chemotaxis, cell adhesion and multidrug resistance.
Application: fluorescent microscopy and flow cytometry.
Properties and Photophysical Data: in live cells, non-fluorescent Calcein AM is hydrolyzed by intracellular esterases into green-fluorescent calcein, which is retained in the cytoplasm. Excitation and emission maxima (λ) are 495 nm and 515 nm, respectively.
It is recommended to prepare stock solutions of Calcein AM in DMSO.
Optical Data
| Emission Color | Green |
| λabs | 495 nm |
| λem | 515 nm |
| Cell Permeable | Yes |
| Application | Flow Cytometry, Fluorescent Microscopy |
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Product Specifications
Molecular Weight
994.86
Formula
C46H46N2O23
Storage
Store at -20°C
Purity
≥90% (HPLC)
Chemical Name
N,N'-[[3',6'-Bis(acetyloxy)-oxospiro[isobenzofuran-1-(3H),9'-(9H)xanthene]-4',5'-diyl]bis(methylene)]bis[N-[2-(acetyloxy)methoxy]-2-oxoethyl]glycine 1,1'-bis[(acetyloxy)methyl] ester
CAS Number
890090-35-4
PubChem ID
4126474
InChI Key
XKFSBWQWNMZWFA-UHFFFAOYSA-N
SMILES
O=C(C)OC3=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C2=C(C=C3)C(C(C=CC=C5)=C5C(O4)=O)4C1=CC=C(OC(C)=O)C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C1O2
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product.
- De Gendt The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay. Clin.Chim.Acta. 1996 PMID: 8737602
- Lazarowski Direct demonstration of mechanically induced release of cellular UTP and its implication for uridine nucleotide receptor activation. J.Biol.Chem. 1997 PMID: 9305892
- Kuehn Prostaglandin E2 activates and utilizes mTORC2 as a central signaling locus for the regulation of mast cell chemotaxis and mediator release. J.Biol.Chem. 2011 PMID: 20980255
- Bakos Membrane topology and glycosylation of the human multidrug resistance-associated protein. J.Biol.Chem. 1996 PMID: 8647833
Product Specific Notices for Calcein AM
For research use only
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