BI dihydrochloride
Tocris Bioscience, part of Bio-Techne | Catalog # 7466
DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers
Key Product Details
Description
DFHBI derivative for imaging of RNA in living cells that bind Broccoli aptamers
Product Description
BI is a DFHBI derivative for imaging of RNA in cells. BI increases brightness and photostability of Broccoli aptamers in cells. BI enables imaging of single Broccoli-tagged mRNAs in living cells. BI exhibits peak excitation of 470 nm and peak fluorescence emission of 505 nm when bound to Broccoli. BI exhibits higher affinity for Broccoli aptamers and a 10.5-fold increase in cellular fluorescence compared with DFHBI-1T (Cat. No. 5610) (KD values are 51 and 305 nM, respectively).Licensing Information
Sold under license from LucernaOptical Data
| Emission Color | Green |
| λabs | 470 nm |
| λem | 505 nm |
| Extinction Coefficient (ε) | 33600 M-1cm-1 |
| Quantum Yield (φ) | 0.669 |
| Cell Permeable | Yes |
| Application | DFHBI Derivative for Imaging of RNA in Living Cells |
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Product Specifications
Molecular Weight
441.26
Formula
C19H14F2N4O2.2HCl
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
(5Z)-3-(1H-Benzimidazol-7-ylmethyl)-5-[(3,5-difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-4H-imidazol-4-one dihydrochloride
CAS Number
2758438-31-0
InChI Key
YKXWKZLDMGAVJO-ZYMKCKPMSA-N
SMILES
CC1=N/C(C(N1CC2=C3NC=NC3=CC=C2)=O)=C\C4=CC(F)=C(C(F)=C4)O.Cl.Cl
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 44.13 | 100 | |
| DMSO | 22.06 | 50 |
Preparing Stock Solutions
for BI dihydrochloride
The following data is based on the product molecular weight 441.26
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.27 mL | 11.33 mL | 22.66 mL |
| 5 mM | 0.45 mL | 2.27 mL | 4.53 mL |
| 10 mM | 0.23 mL | 1.13 mL | 2.27 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.45 mL |
Calculators
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Background References
References are publications that support the biological activity of the product.
- Li Fluorophore promoted RNA folding and photostability enables imaging of single Broccoli-tagged mRNAs in live mammalian cells. Angew.Chem.Int.Ed.Engl. 2020 PMID: 31850609
Product Specific Notices for BI dihydrochloride
For research use only
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