Mouse IFN-gamma ELISpot Kit Best Seller

Name: Mouse IFN-gamma ELISpot Kit
Brand: R&D Systems
SKU: EL485
Rating: 5 (1 reviews)

Catalog # EL485 | R&D Systems, Inc. a Bio-Techne Brand

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Citations (43)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Type:	Quantitative Sandwich ELISA
Format:	96-well PVDF-backed microplate
Assay Length:	3 hours 15 mins to 4 hours 30 mins*
Sample Types:	Whole Cells
Specificity:	Please see the product datasheet

View Full Technical Details for Mouse IFN-gamma ELISpot Kit

Technical Data
Citations (43) / Reviews (1)

Product Summary for Mouse IFN-gamma ELISpot Kit

ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IFN-gamma. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IFN-gamma, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IFN-gamma. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IFN-gamma secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.

Product Features for Mouse IFN-gamma ELISpot Kit

Detect and quantitate individual cells secreting mouse IFN-gamma
High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
No in vitro expansion of cells required
High-throughput - ELISpot assays use only a small number of primary cells

Kit Contents for Mouse IFN-gamma ELISpot Kit

Mouse IFN-gamma Microplate
Biotinylated Detection Antibody
Streptavidin conjugated to Alkaline Phosphatase
Dilution Buffers
Wash Buffer Concentrate
BCIP/NBT Chromogen
Mouse IFN-gamma Positive Control

Other Reagents Required

Pipettes and pipette tips
Deionized or distilled water
Squirt bottle, manifold dispenser, or automated microplate washer
500 mL graduated cylinder
37 °C CO₂ incubator
Sterile culture media
Dissection microscope or an automated ELISpot reader

Formulation, Preparation and Storage

Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Item	Value
Long Name	Interferon gamma
Entrez Gene IDs	3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names	IFG, IFI, IFN-gamma, IFNG, IFNgamma, Immune interferon, interferon gamma, interferon, gamma

Citations for Mouse IFN-gamma ELISpot Kit (43)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse IFN-gamma ELISpot Kit include:

Species: Mouse
Sample Types: Cell Culture Supernates

Ko et al. (2023-10-02), The role of zinc sulfate in enhancing cellular and humoral immune responses to foot-and-mouth disease vaccine Virus research
PMID: 37536380
Species: Mouse
Sample Types: Whole Cells

Wang et al. (2023-06-23), A SARS-CoV-2 and influenza double hit vaccine based on RBD-conjugated inactivated influenza A virus Science advances
PMID: 37352360
Species: Mouse
Sample Types: Whole Cells

MÁ Muñoz-Alía et al. (2022-12-16), Surface-modified measles vaccines encoding oligomeric, fusion-stabilized SARS-CoV-2 spike glycoproteins bypass measles seropositivity, boosting neutralizing antibody responses to omicron and historical variants bioRxiv : the preprint server for biology, 2022-10(0).
PMID: 36561187
Species: Mouse
Sample Types: Tissue Homgenates

JA Lim et al. (2022-12-08), Suppression of pullulanase-induced cytotoxic T cell response with a dual promoter in GSD IIIa mice JCI Insight, 2022-17(23).
PMID: 36264632
Species: Mouse
Sample Types: Cell Culture Supernates

SN Shchelkuno et al. (2022-06-30), Enhancing the Immunogenicity of Vaccinia Virus Viruses, 2022-014(7).
PMID: 35891430
Species: Mouse
Sample Types: Whole Cells

Y Hong et al. (2022-03-08), Cure of syngeneic carcinomas with targeted IL-12 through obligate reprogramming of lymphoid and myeloid immunity JCI Insight, 2022-07(5).
PMID: 35260537
Species: Mouse
Sample Types: Whole Cells

S Klingler et al. (2022-03-08), Disruption of the crypt niche promotes outgrowth of mutated colorectal tumor stem cells JCI Insight, 2022-07(5).
PMID: 35260534
Species: Mouse
Sample Types: Whole Cells

MB Borgoyakov et al. (2022-02-16), Self-Assembled Particles Combining SARS-CoV-2 RBD Protein and RBD DNA Vaccine Induce Synergistic Enhancement of the Humoral Response in Mice International Journal of Molecular Sciences, 2022-023(4).
PMID: 35216301
Species: Mouse
Sample Types: Whole Cells

H Okada et al. (2022-02-08), In situ vaccination using unique TLR9 ligand K3-SPG induces long-lasting systemic immune response and synergizes with systemic and local immunotherapy Scientific Reports, 2022-012(1):2132.
PMID: 35136110
Species: Mouse
Sample Types: Whole Cells

R Uraki et al. (2021-12-09), Foxp3+ CD4+ regulatory T cells control dendritic cells in inducing antigen-specific immunity to emerging SARS-CoV-2 antigens PloS Pathogens, 2021-117(12):e1010085.
PMID: 34882757
Species: Mouse
Sample Types: Whole Cells

W Jiang et al. (2021-11-24), A two-adjuvant multiantigen candidate vaccine induces superior protective immune responses against SARS-CoV-2 challenge Cell Reports, 2021-137(11):110112.
PMID: 34863353
Species: Mouse
Sample Types: Whole Cells

SN Shchelkuno et al. (2021-08-17), Adaptive Immune Response to Vaccinia Virus LIVP Infection of BALB/c Mice and Protection against Lethal Reinfection with Cowpox Virus Viruses, 2021-013(8).
PMID: 34452494
Species: Mouse
Sample Types: Whole Cells

HS Hwang et al. (2020-11-07), Combination of Photodynamic Therapy and a Flagellin-Adjuvanted Cancer Vaccine Potentiated the Anti-PD-1-Mediated Melanoma Suppression Cells, 2020-19(11).
PMID: 33171765
Species: Mouse
Sample Types: Whole Cells

JY Dubé et al. (2020-04-03), Synthetic mycobacterial molecular patterns partially complete Freund's adjuvant Sci Rep, 2020-010(1):5874.
PMID: 32246076
Species: Mouse
Sample Types: Whole Cells

T Aligholipo et al. (2019-09-16), Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models Vaccines (Basel), 2019-07(3).
PMID: 31527460
Species: Mouse
Sample Types: Whole Cells

J Deng et al. (2019-09-04), Broad and Effective Protection against Staphylococcus aureus Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens mSphere, 2019-04(5).
PMID: 31484738
Species: Mouse
Sample Types: Whole Cells

AH Mohseni et al. (2019-01-24), Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis BMC Biotechnol., 2019-019(1):8.
PMID: 30678667
Species: Mouse
Sample Types: Whole Cells

E Gage et al. (2018-12-21), Memory CD4+ T cells enhance B cell responses to drifting influenza immunization Eur. J. Immunol., 2018-10(0).
PMID: 30548475
Species: Mouse
Sample Types: Whole Cells

S Taghinezha et al. (2018-11-02), Protection against human papillomavirus type 16-induced tumors in C57BL/6 mice by mucosal vaccination with Lactococcus lactis NZ9000 expressing E6 oncoprotein Microb. Pathog., 2018-10(0).
PMID: 30391536
Species: Mouse
Sample Types: Whole Cells

C Xu et al. (2018-04-25), Live attenuated Salmonella typhimurium vaccines delivering SaEsxA and SaEsxB via type III secretion system confer protection against Staphylococcus aureus infection BMC Infect. Dis., 2018-018(1):195.
PMID: 29699491
Species: Mouse
Sample Types: Whole Cells

W Sun et al. (2018-01-29), A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses Cell. Mol. Immunol., 2018-00(0).
PMID: 29375131
Species: Mouse
Sample Types: Whole Cells

(2016-12-20), Immunogenicity and Protective Efficacy of the DAR-901 Booster Vaccine in a Murine Model of Tuberculosis PLoS ONE, 2016-111(12):e0168521.
PMID: 27997597
Species: Mouse
Sample Types: Whole Cells

(2016-08-10), Transient Expression of Transgenic IL-12 in Mouse Liver Triggers Unremitting Inflammation Mimicking Human Autoimmune Hepatitis J Immunol, 2016-00(0).
PMID: 27511737
Species: Mouse
Sample Types: Whole Cells

King T et al. (2014-07-22), A whole recombinant yeast-based therapeutic vaccine elicits HBV X, S and Core specific T cells in mice and activates human T cells recognizing epitopes linked to viral clearance. PLoS ONE, 2014-09(7):e101904.
PMID: 25051027
Species: Mouse
Sample Types: Cell Culture Supernates

Reynolds C et al. (2014-05-09), Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile. BMC Biol, 2014-012(0):32.
PMID: 24886643
Species: Mouse
Sample Types: Whole Cells

Kumari S et al. (2014-04-16), Epidermal RelA specifically restricts contact allergen-induced inflammation and apoptosis in skin. J Invest Dermatol, 2014-0134(10):2541-50.
PMID: 24739902
Species: Mouse
Sample Types: Whole Cells

Kanagavelu et al. (2014-02-28), HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity. PLoS ONE, 2014-09(2):e90100.
PMID: 24587225
Species: Mouse
Sample Types: Whole Cells

Lv L et al. (2013-12-27), Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus. J Vet Sci, 2013-115(2):209-16.
PMID: 24378590
Species: Mouse
Sample Types: Whole Cells

Gupta et al. (2013-11-13), Vaccination with a fusion protein that introduces HIV-1 gag antigen into a multitrimer CD40L construct results in enhanced CD8+ T cell responses and protection from viral challenge by vaccinia-gag. J Virol, 2013-188(3):1492-501.
PMID: 24227853
Species: Mouse
Sample Types: Tissue Homogenates

Walsh et al. (2013-03-12), Val-boroPro accelerates T cell priming via modulation of dendritic cell trafficking resulting in complete regression of established murine tumors. PLoS ONE, 2013-08(3):e58860.
PMID: 23554941
Species: Mouse
Sample Types: Whole Cells

Savino et al. (2013-01-23), The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development. J Leukoc Biol, 2013-093(4):549-59.
PMID: 23345394
Species: Mouse
Sample Types: Whole Cells

Wu et al. (2012-08-07), A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PLoS ONE, 2012-07(8):e42363.
PMID: 22879951
Species: Mouse
Sample Types: Whole Cells

Molder T et al. (2009-10-29), Elicitation of broad CTL response against HIV-1 by the DNA vaccine encoding artificial multi-component fusion protein MultiHIV--study in domestic pigs. Vaccine, 2009-128(2):293-8.
PMID: 19879232
Species: Mouse
Sample Types: Whole Cells

Robinson M et al. (2009-02-04), Novel immunocompetent murine tumor model for evaluation of conditionally replication-competent (oncolytic) murine adenoviral vectors. J. Virol., 2009-083(8):3450-62.
PMID: 19193803
Species: Mouse
Sample Types: Whole Cells

Divangahi M et al. (2008-11-15), NOD2-deficient mice have impaired resistance to Mycobacterium tuberculosis infection through defective innate and adaptive immunity. J. Immunol., 2008-1181(10):7157-65.
PMID: 18981137
Species: Mouse
Sample Types: Whole Cells

Lopez R et al. (2008-10-01), Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes. J. Immunol., 2008-1181(7):4908-17.
PMID: 18802094
Species: Mouse
Sample Types: Whole Cells

Yang J et al. (2008-01-22), Critical role of donor tissue expression of programmed death ligand-1 in regulating cardiac allograft rejection and vasculopathy. Circulation, 2008-0117(5):660-9.
PMID: 18212277
Species: Mouse
Sample Types: Whole Cells

Zhu Q et al. (2008-01-01), Immunization with adenovirus at the large intestinal mucosa as an effective vaccination strategy against sexually transmitted viral infection. Mucosal Immunol, 2008-01(1):78-88.
PMID: 19079163
Species: Mouse
Sample Types: Whole Cells

Koncz A et al. (2007-11-15), Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice. J. Immunol., 2007-1179(10):6613-9.
PMID: 17982051
Species: Mouse
Sample Types: Whole Cells

Barcia C et al. (2007-09-25), One-year Expression From High-capacity Adenoviral Vectors in the Brains of Animals With Pre-existing Anti-adenoviral Immunity: Clinical Implications. Mol. Ther., 2007-015(12):2154-2163.
PMID: 17895861
Species: Mouse
Sample Types: Whole Cells

Kurooka M et al. (2007-01-01), Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells. Cancer Res., 2007-067(1):227-36.
PMID: 17210703
Species: Mouse
Sample Types: Whole Cells
Applications: ELISpot Development

Young KR et al. (2006-01-01), Elicitation of immunity to HIV type 1 Gag is determined by Gag structure. AIDS Res. Hum. Retroviruses, 2006-022(1):99-108.
PMID: 16438652
Species: Mouse
Sample Types: Whole Cells

Wang D et al. (2004-04-01), Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs. Int. J. Oncol., 2004-024(4):901-8.
PMID: 15010828

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Mouse IFN-gamma ELISpot Kit

Name: Anonymous

Sample Tested: Mouse splenocytes

Verified Customer | Posted 10/27/2016

http://pubs.acs.org/doi/abs/10.1021/jacs.6b05675

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Product Documents for Mouse IFN-gamma ELISpot Kit

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⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.