Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-31035
Key Product Details
Sample Type & Volume Required Per Well
Serum, plasma, tissue homogenates and other biological fluids (100 uL)
Sensitivity
0.121 ng/mL (example only; lot dependent)
Assay Range
0.312 - 20 ng/mL (example only; lot dependent)
Product Specifications
Assay Type
Sandwich ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human
Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Assay Length: 3 hours
Precision
Intra-Assay Precision (Precision within an assay) %CV < 10 (example only; lot dependent)
Inter-Assay Precision (Precision between assays) %CV < 12 (example only; lot dependent)
Scientific Data Images for Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric)
ELISA: Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31035] -
ELISA: Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31035] - Standard Curve ReferenceKit Contents for Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric)
- Detection Solution A
- Detection Solution B
- Instruction manual
- Plate sealer for 96 wells
- Pre-coated 96T strip plate
- Standard
- Standard Diluent
- Stop Solution
- TMB Substrate
- Wash Buffer (30 x concentrate)
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: TRACP/PAP/ACP5
TRACP is an iron-containing, 35 kDa glycoprotein that hydrolyses phosphomonoesters. It is produced as a monomer by osteoclasts and is cleaved by cathepsins to create a disulfide-linked dimer that acts on bone matrix phosphoserine-containing proteins in lysosomal vesicles. Following phosphate cleavage, TRACP is released from the cell where it serves as a marker of bone resorption.
Long Name
Tartrate Resistant Acid Phosphatase 5
Alternate Names
ACP5, TRACP-5b, TTRRAP
Gene Symbol
ACP5
Additional TRACP/PAP/ACP5 Products
Product Documents for Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric)
Product Specific Notices for Human TRACP/PAP/ACP5 - Ready-To-Use ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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