Tankyrase 1 Colorimetric Activity Assay

The Tankyrase 1 Colorimetric Activity Assay is a high-throughput, semi-quantitative ELISA designed to measure Tankyrase 1 activity. Tankyrase 1, also known as PARP5a, is a Poly (ADP-ribose) Polymerase (PARP) that associates with telomeres via its interaction with the telomeric-repeat binding factor TRF1. This assay utilizes a histone-coated 96-well microplate and an anti-poly (ADP-ribose) (PAR) antibody to detect PAR that has been deposited onto the immobilized histone proteins by Tankyrase 1. The Tankyrase 1 Colorimetric Activity Assay uses TACS-Sapphire^® as the colorimetric substrate. This is the only commercially available assay to feature a full length Tankyrase 1 enzyme and unmodified NAD substrate to create a more physiologically applicable in vitro assay system.

Features

• Amenable to high-throughput
• Highly sensitive: capable of measuring 0.1 mUnits of Tankyrase 1
• 3 hour assay time
• Provides full length Tankyrase 1 enzyme and unmodified NAD substrate

Kit Contents

• Histone-coated 96-stripwell Microplate
• Anti-PAR Monoclonal Antibody
• HRP-conjugated Goat Anti-Mouse Antibody
• Tankyrase 1
• XAV939^a
• Diluents & Buffers
• Unmodified NAD Substrate
• HRP Substrate

^a XAV939 is a Tankyrase 1 inhibitor
TACS-Sapphire is a registered trademark of Trevigen, Inc.

Citations

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