Smith Antigen ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # KA1112
Key Product Details
Sample Type & Volume Required Per Well
Plasma, Serum (100 uL (prediluted))
Sensitivity
1.0 U/mL
Assay Range
12.5 to 200 U/mL
Product Specifications
Assay Type
Indirect ELISA
Kit Type
ELISA Kit (Colorimetric)
Description
Sm Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against Sm in human serum or plasma.
Precision
Intra-Assay Precision (Precision within an assay) CV% < 2.0-4.1%
Inter-Assay Precision (Precision between assays) CV% < 1.3-5.3%
Kit Contents for Smith Antigen ELISA Kit (Colorimetric)
- Calibrators A-F (0, 12.5, 25, 50, 100, 200 U/mL), containing Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use.
- Control positive (1) and negative (2), containing Sm antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN3 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
- Divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
- Enzyme conjugate, containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative Proclin 300 0.05%, light red. Ready to use.
- Sample buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, concentrate (5x).
- Stop solution, contains acid. Ready to use.
- TMB substrate, containing 3,3’,5,5’- Tetramethylbenzidine, colorless. Ready to use.
- Wash solution, containing Tris, detergent, preservative NaN3 0.09%, 50x concentrate.
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Smith Antigen
Alternate Names
Smith antigen
Additional Smith Antigen Products
Product Documents for Smith Antigen ELISA Kit (Colorimetric)
Product Specific Notices for Smith Antigen ELISA Kit (Colorimetric)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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