Rat TIMP-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RTM100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL)
Sensitivity
4.5 pg/mL
Assay Range
37.5-2400 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Rat TIMP-1 Quantikine ELISA Kit
The Quantikine Rat TIMP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat TIMP-1 levels in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant rat TIMP-1 and antibodies raised against the recombinant protein. Natural rat TIMP-1 showed dose-response curves that were parallel to the standard curves obtained using the Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural rat TIMP-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat TIMP-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 122 | 443 | 1307 | 132 | 445 | 1277 |
| Standard Deviation | 4.5 | 14 | 34 | 9.6 | 27 | 85 |
| CV% | 3.7 | 3.2 | 2.6 | 7.3 | 6.1 | 6.7 |
Recovery for Rat TIMP-1 Quantikine ELISA Kit
The recovery of rat TIMP-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 106 | 93-119 |
| EDTA Plasma (n=7) | 99 | 82-115 |
| Serum (n=7) | 98 | 84-120 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with rat TIMP-1 were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Rat TIMP-1 Quantikine ELISA Kit
Rat TIMP-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIMP-1
TIMP-1 (tissue inhibitor of metalloproteinase-1) is a secreted glycoprotein that blocks metalloproteinase (MMP) activity. It circulates in complex with various MMPs to maintain them in an inactive state. It is produced by fibroblasts, osteoblasts, endothelial cells, granulosa cells, dendritic cells, vascular smooth muscle cells, adipocytes, and monocytes. TIMP-1 also protects against apoptosis in B cells, stimulates erythropoiesis and BFU-E expansion, both stimulates and inhibits tumor growth, inhibits angiogenesis, and stimulates gonadal steroidogenesis in conjuction with Cathepsin L.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Alternate Names
TIMP1
Gene Symbol
TIMP1
Additional TIMP-1 Products
Product Documents for Rat TIMP-1 Quantikine ELISA Kit
Product Specific Notices for Rat TIMP-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.