Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RKM100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Urine (25 µL)
Sensitivity
2.74 pg/mL
Assay Range
7.81-500 pg/mL (Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma, Urine)
Product Summary for Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
The Quantikine Rat TIM-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat TIM-1 in cell culture supernates, tissue homogenates, serum, plasma, and urine. It contains NS0-expressed recombinant rat TIM-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat TIM-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat TIM-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat TIM-1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 21.8 | 54.4 | 161 | 26.2 | 60.0 | 169 |
| Standard Deviation | 0.80 | 2.45 | 5.22 | 2.32 | 3.79 | 7.51 |
| CV% | 3.7 | 4.5 | 3.2 | 8.9 | 6.3 | 4.4 |
Recovery for Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
The recovery of rat TIM-1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=5) | 96 | 93-99 |
| EDTA Plasma (n=4) | 99 | 84-107 |
| Heparin Plasma (n=4) | 102 | 95-114 |
| Serum (n=4) | 102 | 82-112 |
| Urine (n=4) | 103 | 87-117 |
Linearity
To assess the linearity of the assay, samples containing or spiked with rat TIM-1 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Rat TIM-1/KIM-1/HAVCR ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TIM-1/KIM-1/HAVCR
TIM-1 (T cell immunoglobulin and mucin domain 1), also known as KIM-1 and HAVcr1, is expressed on many immune cell types and epithelial cells. It binds to phosphatidylserine (PS), LMIR5/CD300b, TIM-1 (homophilic), TIM-4, IgA, and the glycoproteins of a number of enveloped viruses. Its interaction with PS enables TIM-1 to mediate the phagocytosis and clearance of apoptotic cells. TIM-1 signaling costimulates T cell activation and enhances Th2 cytokine production. TIM-1 serves as a cellular entry receptor for hepatitis A virus, Ebolavirus and Marburgvirus. Polymorphisms are associated with susceptibility to atopy, autoimmunity, and severe hepatitis A virus infection in humans. A soluble form of TIM-1 is elevated in the urine during nephropathy.
Long Name
T Cell Immunoglobulin Mucin-1
Alternate Names
CD365, HAVCR1, KIM-1, TIM1
Gene Symbol
HAVCR1
Additional TIM-1/KIM-1/HAVCR Products
Product Documents for Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
Product Specific Notices for Rat TIM-1/KIM-1/HAVCR Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.