Rat CXCL1/CINC-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # RCN100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
Sensitivity
1.3 pg/mL
Assay Range
7.8-500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Rat CXCL1/CINC-1 Quantikine ELISA Kit
The Quantikine Rat CINC-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure rat CINC-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant rat CINC-1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural rat CINC-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring rat CINC-1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Rat
Specificity
Natural and recombinant rat CINC-1
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 23 | 23 | 23 |
| Mean (pg/mL) | 53 | 71 | 215 | 49 | 71 | 190 |
| Standard Deviation | 2.7 | 5.2 | 12 | 3.1 | 3.3 | 12 |
| CV% | 5.1 | 7.3 | 5.6 | 6.3 | 4.6 | 6.3 |
Recovery for Rat CXCL1/CINC-1 Quantikine ELISA Kit
The recovery of rat CINC-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 99 | 93-106 |
| EDTA Plasma (n=4) | 97 | 90-104 |
| Heparin Plasma (n=4) | 99 | 90-113 |
| Serum (n=5) | 95 | 89-103 |
Linearity
To assess the linearity of the assay, samples containing rat CINC-1 were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Rat CXCL1/CINC-1 Quantikine ELISA Kit
Rat CXCL1/GRO alpha/KC/CINC-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: CXCL1/GRO alpha/KC/CINC-1
CXCL1/GRO alpha/KC/CINC-1 is an ELR+ angiogenic chemokine that interacts with CXCR2/IL-8 RB to attract and activate neutrophils. It is produced at sites of microbial infection by macrophages, monocytes, Kupffer cells, hepatocytes, endothelial and epithelial cells, cardiac and vascular smooth muscle cells, fibroblasts, melanocytes, mast cells, and neurons. CXCL1 function is associated with the development and extent of atherosclerosis, Alzheimer’s disease, and tumor angiogenesis.
Alternate Names
CINC-1, CINC1, GRO alpha, KC, MGSA-alpha
Gene Symbol
CXCL1
Additional CXCL1/GRO alpha/KC/CINC-1 Products
Product Documents for Rat CXCL1/CINC-1 Quantikine ELISA Kit
Product Specific Notices for Rat CXCL1/CINC-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.