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Sheep Progesterone ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60121

Novus Biologicals, part of Bio-Techne
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NBP2-60121-1Kit

Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Sheep

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Scientific Data Images for Sheep Progesterone ELISA Kit (Colorimetric)

ELISA: Sheep Progesterone ELISA Kit (Colorimetric) [NBP2-60121]

ELISA: Sheep Progesterone ELISA Kit (Colorimetric) [NBP2-60121]

ELISA: Sheep Progesterone ELISA Kit [NBP2-60121] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Sheep Progesterone ELISA Kit (Colorimetric)

Sheep Progesterone ELISA Kit (Colorimetric) [NBP2-60121] -

Sheep Progesterone ELISA Kit (Colorimetric) [NBP2-60121] - Standard Curve Reference

Kit Contents for Sheep Progesterone ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antibody
  • Assay plate
  • HRP-conjugate
  • Instruction manual
  • Standard
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (20 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Progesterone

Progesterone plays a central role in the reproductive events associated with the establishment and maintenance of pregnancy. Progesterone receptor, a member of the steroid receptor superfamily, mediates the physiologic effects of progesterone. The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165 amino acids present only in the N terminus of PRB. Although PRA and PRB share several structural domains, they are distinct transcription factors that mediate their own response genes and physiologic effects with little overlap. It is composed of three domains: a modulating N terminal domain, a DNA binding domain and a C terminal steroid binding domain. Progesterone levels 1. men 30-60 pg/0.1ml 2. women pre ovulatory phase: 20-160 pg/0.1ml; ovulatory phase: 1,000-1,700 pg/0.1ml; post ovulatory phase: 1,000-1,700 pg/0.1ml; Pregnant: 16-18 weeks: 300-800 pg/0.1ml; 28-30 weeks: 6,500-14,700 pg/0.1ml; 38-40 weeks: 12,000-19,000 pg/0.1ml.

Alternate Names

4-Pregnen-3,20-dione

Additional Progesterone Products

Product Documents for Sheep Progesterone ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Sheep Progesterone ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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