Human Nuclear Autoantibody ELISA Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59873

Novus Biologicals, part of Bio-Techne
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Key Product Details

Sample Type & Volume Required Per Well

Serum (50 - 100 uL)

View all Nuclear Autoantibody ELISA Kits »

Product Specifications

Assay Type

Indirect ELISA

Kit Type

ELISA Kit (Colorimetric)

Reactivity

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human Nuclear Autoantibody. No significant cross-reactivity or interference between human Nuclear Autoantibody and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV < 15

Inter-Assay Precision (Precision between assays) %CV < 15

Kit Contents for Human Nuclear Autoantibody ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Coated assay plate
  • HRP-conjugate
  • Instruction manual
  • Negative Control
  • Positive Control
  • Sample Diluent
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Nuclear Autoantibody

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human immunoglobulin conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human Nuclear Autoantibody bound in the initial step. The color development is stopped and the intensity of the color is measured.

Alternate Names

ANA ELISA, Anti-Nuclear Antibody, Nuclear Antibody ELISA

Additional Nuclear Autoantibody Products

Product Documents for Human Nuclear Autoantibody ELISA Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Human Nuclear Autoantibody ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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