Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MVR100
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL)
Sensitivity
15.2 pg/mL
Assay Range
125-8000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Product Summary for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
The Quantikine Mouse soluble VEGF R1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R1 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse sVEGF R1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R1.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse sVEGF R1
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 265 | 1383 | 2174 | 250 | 1339 | 2139 |
| Standard Deviation | 19 | 55 | 69 | 21 | 96 | 134 |
| CV% | 7.2 | 4.0 | 3.2 | 8.4 | 7.2 | 6.3 |
Recovery for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
The recovery of mouse sVEGF R1 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=6) | 96 | 82-110 |
| EDTA Plasma (n=6) | 106 | 92-120 |
| Serum (n=6) | 106 | 96-119 |
Linearity
To assess the linearity of the assay, six samples containing and/or spiked with high concentrations of mouse sVEGF R1 in each matrix were diluted with Calibrator Diluent and then assayed.
Scientific Data Images for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
Mouse VEGF R1/Flt-1 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: VEGFR1/Flt-1
VEGFR1 (vascular endothelial growth factor receptor 1; Flt-1) binds to VEGF-A, VEGF-B, and PlGF and functions in vasculogenesis, angiogenesis, lymphangiogenesis, and wound healing. It is expressed on endothelial cells, osteoblasts, placental trophoblasts, renal mesangial cells, and some hematopoietic stem cells. It can heterodimerize with VEGFR2 and modulate VEGFR2 mediated endothelial cell proliferation and vessel branching. VEGFR1 engagement on monocyte/macrophage lineage cells enhances their migration, and release of growth factors and cytokines.
Long Name
Vascular Endothelial Growth Factor Receptor 1
Alternate Names
Flt-1, FLT1, FRT, VEGF R1, VEGFR-1
Gene Symbol
FLT1
Additional VEGFR1/Flt-1 Products
Product Documents for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
Product Specific Notices for Mouse VEGFR1/Flt-1 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.