Mouse/Rat TNF RI/TNFRSF1A Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MRT10
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (10 µL)
Sensitivity
2.4 pg/mL
Assay Range
15.6-1000 pg/mL (Cell Culture Supernates, Serum)
Product Summary for Mouse/Rat TNF RI/TNFRSF1A Quantikine ELISA Kit
The Quantikine Mouse/Rat TNF RI immunoassay is a 4.5 hour solid-phase ELISA designed to measure TNF RI in mouse or rat cell culture supernates and serum. It contains E. coli-expressed recombinant mouse TNF RI and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse TNF RI. Results obtained using natural mouse or rat TNF RI showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural TNF RI.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat TNF RI
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 46.0 | 118 | 581 | 48.3 | 120 | 592 |
| Standard Deviation | 2.7 | 6.5 | 27.5 | 2.5 | 6.0 | 33.3 |
| CV% | 5.9 | 5.5 | 4.7 | 5.2 | 5.0 | 5.6 |
Recovery for Mouse/Rat TNF RI/TNFRSF1A Quantikine ELISA Kit
The recovery of TNF RI spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=5) | 97 | 89-109 |
| Mouse Serum (n=5) | 96 | 83-104 |
| Rat Cell Culture Supernates (n=5) | 98 | 92-116 |
| Rat Serum (n=5) | 96 | 88-104 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of TNF RI in each matrix were diluted with Calibrator Diluent and assayed.
Scientific Data Images for Mouse/Rat TNF RI/TNFRSF1A Quantikine ELISA Kit
Mouse/Rat TNF RI/TNFRSF1A ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: TNF RI/TNFRSF1A
TNF RI (TNF receptor 1; TNF R-p55/p60) is a transmembrane receptor for TNF-alpha and Lymphotoxin-alpha/TNF-beta. A soluble decoy form of the receptor is shed by ADAM-17/TACE in response to inflammatory stimulation. TNF RI signaling through NFkB is essential for the development of lymph node germinal centers and Peyer’s patches and for combating intracellular pathogens such as Listeria.
Long Name
Tumor Necrosis Factor Receptor I
Alternate Names
CD120a, TNFRI, TNFRSF1A
Gene Symbol
TNFRSF1A
Additional TNF RI/TNFRSF1A Products
Product Documents for Mouse/Rat TNF RI/TNFRSF1A Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat TNF RI/TNFRSF1A Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.