Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MANG20
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
4.98 pg/mL
Assay Range
39.1-2500 pg/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
The Quantikine Mouse/Rat Angiopoietin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Angiopoietin-2 in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse Angiopoietin-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural Angiopoietin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural Angiopoietin-2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse, Rat
Specificity
Natural and recombinant mouse and rat Angiopoietin-2.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 119 | 290 | 831 | 116 | 283 | 841 |
| Standard Deviation | 3.27 | 9.05 | 22.2 | 9.84 | 23.2 | 63.1 |
| CV% | 2.7 | 3.1 | 2.7 | 8.5 | 8.2 | 7.5 |
Recovery for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 114 | 108-120 |
| EDTA Plasma (n=2) | 105 | 101-110 |
| Heparin Plasma (n=2) | 107 | 103-111 |
| Serum (n=2) | 113 | 109-117 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
Mouse/Rat Angiopoietin-2 ELISA Standard Curve
Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
Endothelial permeability in response to CLP. Plasma levels of endocan (A) and angiopoietin‐2 (B), soluble markers of endothelial permeability were measured in Sham and CLP rats (n = 8/group) via ELISA. *P < 0.05 versus Sham. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31054188), licensed under a CC-BY license. Not internally tested by R&D Systems.Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Angiopoietin-2
Angiopoietin-2 (Ang-2; also ANGPT2) is a secreted glycoprotein that regulates vascular remodeling by promoting endothelial cell survival, proliferation, and migration and destabilizing the interaction between EC and perivascular cells. Ang-2 is required for postnatal vascular remodeling, and it cooperates with Ang-1 during lymphatic vessel development. It mediates the upregulation of ICAM-1 and VCAM-1 on EC, which facilitates the adhesion of leukocytes during inflammation. Its direct interaction with Integrins promotes tumor cell invasion. Ang-2 is widely expressed during development, but it is restricted postnatally to highly angiogenic tissues such as the placenta, ovaries, and uterus. It associates into dimers, trimers, and larger multimers and binds the receptor Tie-2, with its function as an agonist or antagonist depending on its state of multimerization.
Alternate Names
ANGPT2
Entrez Gene IDs
Gene Symbol
ANGPT2
Additional Angiopoietin-2 Products
Product Documents for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
Product Specific Notices for Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.