Mouse IL-17 Quantikine HS ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # MHS170
Key Product Details
Assay Length
4.0 hours
Sample Type & Volume Required Per Well
Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
0.214 pg/mL
Assay Range
0.781-50 pg/mL (Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse IL-17 Quantikine HS ELISA Kit
The Quantikine™ HS Mouse IL-17 Immunoassay is a 4.0 hour solid phase ELISA designed to measure mouse IL-17 levels in serum and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant protein. Results obtained using natural IL-17 showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that the Quantikine HS Immunoassay kit can be used to determine relative mass values for natural mouse IL-17.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450 or 490nm (TMB or AP Substrate)
Conjugate
HRP
Reactivity
Mouse
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 6.82 | 16.9 | 31.9 | 6.92 | 16.6 | 31.4 |
| Standard Deviation | 0.239 | 0.429 | 0.836 | 0.349 | 0.814 | 1.64 |
| CV% | 3.5 | 2.5 | 2.6 | 5.1 | 4.9 | 5.2 |
Recovery for Mouse IL-17 Quantikine HS ELISA Kit
The recovery of mouse IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| EDTA Plasma (n=4) | 90 | 78-96 |
| Heparin Plasma (n=4) | 89 | 80-97 |
| Serum (n=4) | 89 | 75-98 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17 were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse IL-17 Quantikine HS ELISA Kit
Mouse IL-17 Quantikine HS ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-17/IL-17A
IL-17/IL-17A is a homodimeric cytokine that is secreted by IL-23 activated Th17 and Tc17 cells. It is upregulated during inflammation and contributes to disease severity by enhancing neutrophil infiltration and the production of pro-inflammatory chemokines. IL-17A can additionally form bioactive heterodimers with IL-17F. IL-17A and the IL-17A/F heterodimer signal through widely expressed receptor complexes containing IL-17 RA and IL-17 RC.
Long Name
Interleukin 17
Alternate Names
CTLA-8, CTLA8, IL-17A, IL17, IL17A
Entrez Gene IDs
Gene Symbol
IL17A
Additional IL-17/IL-17A Products
Product Documents for Mouse IL-17 Quantikine HS ELISA Kit
Product Specific Notices for Mouse IL-17 Quantikine HS ELISA Kit
For research use only
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