Mouse IL-13 R alpha 2 Quantikine ELISA Kit
R&D Systems, part of Bio-Techne | Catalog # M13RA2
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Homogenates (13 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
5.6 pg/mL
Assay Range
39-2500 pg/mL
Product Summary for Mouse IL-13 R alpha 2 Quantikine ELISA Kit
The Quantikine Mouse IL-13 R alpha2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 R alpha2 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse IL-13 R alpha2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 R alpha2 accurately. Results obtained using natural mouse IL-13 R alpha2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13 R alpha2.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse IL-13 R alpha2
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Homogenates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 97.7 | 338 | 1011 | 101 | 279 | 1008 |
| Standard Deviation | 4.25 | 12.5 | 23.2 | 8.2 | 17.6 | 50.3 |
| CV% | 4.4 | 3.7 | 2.3 | 8.1 | 6.3 | 5.0 |
Recovery for Mouse IL-13 R alpha 2 Quantikine ELISA Kit
The recovery of mouse IL-13 R alpha2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Samples (n=4) | 91 | 83-98 |
| EDTA Plasma (n=4) | 99 | 97-101 |
| Heparin Plasma (n=4) | 103 | 87-117 |
| Serum (n=4) | 97 | 83-108 |
| Tissue Homogenates (n=4) | 106 | 93-119 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-13 R alpha2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse IL-13 R alpha 2 Quantikine ELISA Kit
Mouse IL-13 R alpha 2 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-13 R alpha 2
Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.
Long Name
Interleukin 13 Receptor alpha 2
Alternate Names
CD213a2, IL-13Ra2, IL13R alpha 2, IL13RA2
Gene Symbol
IL13RA2
Additional IL-13 R alpha 2 Products
Product Documents for Mouse IL-13 R alpha 2 Quantikine ELISA Kit
Product Specific Notices for Mouse IL-13 R alpha 2 Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.