Mouse IL-13 R alpha 2 Quantikine ELISA Kit

Name: Mouse IL-13 R alpha 2 Quantikine ELISA Kit
Brand: R&D Systems
SKU: M13RA2

Catalog # M13RA2 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Assay Length:	4.5 hours
Sample Type & Volume Required Per Well:	Cell Culture Supernates (50 uL), Tissue Homogenates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)
Sensitivity:	5.6 pg/mL
Assay Range:	39 - 2,500 pg/mL
Mouse IL-13 R alpha 2 Quantikine ELISA Kit Summary:	The Quantikine Mouse IL-13 R alpha 2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-13 R alpha 2 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse IL-13 R alpha 2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse IL-13 R alpha 2 accurately. Results obtained using natural mouse IL-13 R alpha 2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-13 R alpha 2.

View Full Technical Details for Mouse IL-13 R alpha 2 Quantikine ELISA Kit

Technical Data
Citations (1) / Reviews

Product Specifications for Mouse IL-13 R alpha 2 Quantikine ELISA Kit

Assay Type	Solid Phase Sandwich ELISA
Format	96-well strip plate
Measurement	Quantitative ELISA
Detection Method	Colorimetric - 450nm (TMB)
Conjugate	HRP
Reactivity	Mouse
Specificity	Natural and recombinant mouse IL-13 R alpha 2
Cross-reactivity	< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference	Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	97.7	338	1011	101	279	1008
Standard Deviation	4.25	12.5	23.2	8.2	17.6	50.3
CV%	4.4	3.7	2.3	8.1	6.3	5

Recovery

The recovery of mouse IL-13 R alpha 2 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Samples (n=4)	91	83-98
EDTA Plasma (n=4)	99	97-101
Heparin Plasma (n=4)	103	87-117
Serum (n=4)	97	83-108
Tissue Homogenates (n=4)	106	93-119

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-13 R alpha 2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Examples for Mouse IL-13 R alpha 2 Quantikine ELISA Kit

	Mouse IL-13 R alpha 2 ELISA Standard Curve

Preparation and Storage

Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-13 R alpha 2

Two members of the type 5 subfamily of type I cytokine receptors can serve as receptors for IL-13. IL-13 can bind to IL-13 R alpha 1 (CD213a1; previously designated IL-13 R alpha or NR4) with low affinity, then recruits the IL-4 R alpha chain to form a high affinity receptor, causing downstream STAT6 activation. Alternately, IL-13 can bind IL-13 R alpha 2 (CD213a2) with high affinity; this interaction does not cause activation of STAT6, but does induce TGF-beta production. IL-13 R alpha 1 and IL-13 R alpha 2 each have three extracellular fibronectin type III domains, two cytokine receptor homology modules and a WSXWS motif typical of the class I cytokine receptor family, but IL-13 R alpha 2 has a much shorter cytoplasmic tail. IL-13 R subunits can be expressed on monocytes, macrophages, fibroblasts, human B cells, basophils, eosinophils, endothelial cells, and smooth muscle cells.

Item	Value
Long Name	Interleukin 13 Receptor alpha 2
Entrez Gene IDs	3598 (Human); 16165 (Mouse)
Alternate Names	CD213a2, CD213a2 antigen, CT19, IL-13 R alpha 2, IL-13 receptor subunit alpha-2, IL-13R, IL-13R subunit alpha-2, IL-13R-alpha-2, IL-13Ra2, IL13BP, IL13R, IL13R alpha 2, IL13RA2, Interleukin-13-binding protein, cancer/testis antigen 19, interleukin 13 binding protein, interleukin 13 receptor alpha 2 chain, interleukin 13 receptor, alpha 2, interleukin-13 receptor subunit alpha-2

Citations for Mouse IL-13 R alpha 2 Quantikine ELISA Kit (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse IL-13 R alpha 2 Quantikine ELISA Kit include:

Species: Mouse
Sample Types: Serum

S Bhattachar et al. (2019-07-02), Concomitant Infection of S.ï¿½mansoni and H.ï¿½pylori Promotes Promiscuity of Antigen-Experienced Cells and Primes the Liver for a Lower Fibrotic Response Cell Rep, 2019-028(1):231-244.e5.
PMID: 31269443

There are no citations that match your criteria.

Showing 1 - 1 of 1 citation Showing All

Product Documents for Mouse IL-13 R alpha 2 Quantikine ELISA Kit

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.