Mouse C-Reactive Protein/CRP ELISA Kit - Quantikine
R&D Systems, part of Bio-Techne | Catalog # MCRP00
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Tissue Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
Sensitivity
0.015 ng/mL
Assay Range
0.156-10 ng/mL (Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
The Quantikine Mouse C-Reactive Protein/CRP immunoassay is a 4.5 hour solid-phase ELISA designed to measure CRP in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse CRP and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse CRP. Results obtained using natural mouse CRP showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse CRP.
Product Specifications
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Reactivity
Mouse
Specificity
Natural and recombinant mouse CRP.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum, Tissue Lysates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.453 | 1.31 | 3.78 | 0.480 | 1.33 | 3.76 |
| Standard Deviation | 0.035 | 0.083 | 0.089 | 0.052 | 0.087 | 0.314 |
| CV% | 7.7 | 6.3 | 2.4 | 10.8 | 6.5 | 8.4 |
Recovery for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
The recovery of mouse CRP spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 82-115 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse CRP in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
Mouse C-Reactive Protein/CRP ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: C-Reactive Protein/CRP
C-Reactive Protein (CRP), also known as Pentraxin 1, is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans, it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice, however, serum CRP levels increase only slightly during inflammation, and the analogous acute phase role is filled by Pentraxin 2. CRP binds, opsonizes, and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI, Fc gamma RIIA, and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease, CRP binds to oxidized LDL, exacerbates tissue damage in myocardial infarction, and inhibits the repair of injured vascular endothelium.
Additional C-Reactive Protein/CRP Products
Product Documents for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
Product Specific Notices for Mouse C-Reactive Protein/CRP Quantikine ELISA Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.