Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62161
Key Product Details
Sample Type & Volume Required Per Well
Human, mouse, and rat serum, plasma, urine (100 uL)
Sensitivity
0.087 ng/ml
Assay Range
0.13-1000 ng/ml
Product Specifications
Assay Type
Competitive ELISA
Kit Type
ELISA Kit (Colorimetric)
Reactivity
Human, Mouse, Rat
Precision
Intra-Assay Precision (Precision within an assay) Low Concentration %CV - 20.5% High Concentration %CV - 9.8%
Inter-Assay Precision (Precision between assays) Low Concentration %CV - 22.4% High Concentration %CV - 17.1%
Recovery for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)
Recovery
Human Serum
Low - 71%
Medium - 89%
High - 86%
Human Plasma Low - 91%
Medium - 107%
High - 77%
Mouse Serum Low - 141%
Medium - 140%
High - 135%
Mouse Plasma Low - 90%
Medium - 168%
High - 56%
Medium - 89%
High - 86%
Human Plasma Low - 91%
Medium - 107%
High - 77%
Mouse Serum Low - 141%
Medium - 140%
High - 135%
Mouse Plasma Low - 90%
Medium - 168%
High - 56%
Scientific Data Images for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)
![ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161]](https://resources.bio-techne.com/images/products/Human-Mouse-Rat-Methotrexate-ELISA-Kit-Colorimetric-ELISA-NBP2-62161-img0001.jpg "ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161]")
ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161]
ELISA: Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric) [NBP2-62161] - A typical standard curve for Methotrexate ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.Kit Contents for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)
- and Stop Solution
- Antibody
- Antibody Diluent
- Assay Buffer 13
- Conjugate
- Microtiter Plate
- Standard
- Substrate
- Wash Buffer Concentrate
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4°C.
Background: Methotrexate
Alternate Names
Amethopterine, MTX
Additional Methotrexate Products
Product Documents for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)
Product Specific Notices for Human, Mouse, Rat Methotrexate ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
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