LTB4 Parameter Assay Kit
R&D Systems, part of Bio-Techne | Catalog # KGE006B
Key Product Details
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
Sensitivity
10.9 pg/mL
Assay Range
10.3-2500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Product Summary for LTB4 Parameter Assay Kit
The Parameter LTB4 Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
Biotin
Reactivity
Multi-Species
Specificity
Measures LTB4 levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (pg/mL) | 170 | 481 | 847 | 164 | 516 | 879 |
| Standard Deviation | 10 | 19 | 34 | 14.1 | 36.6 | 73.6 |
| CV% | 5.8 | 4.0 | 4.0 | 8.6 | 7.1 | 8.4 |
Recovery for LTB4 Parameter Assay Kit
The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-114 |
| EDTA Plasma (n=4) | 91 | 82-112 |
| Heparin Plasma (n=4) | 98 | 82-115 |
| Serum (n=4) | 98 | 82-118 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of LTB<SUB>4</SUB> were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for LTB4 Parameter Assay Kit
Multi-species Leukotriene B4/LTB4 ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: Leukotriene B4/LTB4
Alternate Names
LTB4
Additional Leukotriene B4/LTB4 Products
Product Documents for LTB4 Parameter Assay Kit
Product Specific Notices for LTB4 Parameter Assay Kit
For research use only
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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.