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Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit

Bio-Techne includes R&D Systems | Catalog # DSGC10

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DSGC10

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL), Human Milk (10 µL)

Sensitivity

25.6 pg/mL

Assay Range

62.5-2000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk)

Product Summary for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit

The Quantikine Human Serpin G1/C1 Inhibitor Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin G1 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains human Serpin G1 purified from plasma and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin G1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Serpin G1.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Reactivity

Human

Specificity

Natural human Serpin G1.

Cross-reactivity

Interference

No significant interference observed with available related molecules.

Sample Values

Sample TypeMean (ng/mL)Range (ng/mL)
Serum (n=30)138,74655,639-323,345
EDTA Plasma (n=30)136,44676,442-259,277
Heparin (n=30)131,21877,947-225,264
Saliva (n=10)50.511.0-121
Urine (n=10)66.116.9-176
Human Milk (n=10)601238-1318

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Human Milk, Saliva, Serum, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 16 16 16 16 16 16
Mean (pg/mL) 206 634 1209 212 603 1191
Standard Deviation 10.4 24.7 56.5 13.7 31.7 53.4
CV% 5.0 3.9 4.7 6.5 5.3 4.5

Recovery for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit

The recovery of human Serpin G1 spiked to levels throughout the range of the assay in cell culture media was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 90-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin G1 were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.

Scientific Data Images for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit

Human Serpin G1/C1 Inhibitor ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Serpin G1/C1 Inhibitor

The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.

Alternate Names

C1 Inhibitor

Entrez Gene IDs

710 (Human)

Gene Symbol

SERPING1

Additional Serpin G1/C1 Inhibitor Products

Product Documents for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Serpin G1/C1 Inhibitor Quantikine ELISA Kit

For research use only

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